Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

16.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
16.0K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

907
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
907
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.3K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

10.7K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
10.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Asymmetric Chichibabin diradicaloids: synthesis <i>via</i> oxidation of SOMO-HOMO inversion radicals and near-infrared absorption for electrochromism.

Chemical science·2026
Same author

SWIFT: A Small-World Interaction Framework for Flow-Aware Trajectory Prediction in Autonomous Driving.

IEEE transactions on pattern analysis and machine intelligence·2026
Same author

Synthesis and characterization of a π-extended nonbenzenoid perylene.

Chemical communications (Cambridge, England)·2026
Same author

Air-Stable Luminescent Schlenk Diradical With Triplet Ground State.

Angewandte Chemie (International ed. in English)·2026
Same author

Preclinical <i>CRX</i> augmentation therapies for <i>CRX</i>-associated autosomal dominant cone-rod dystrophies.

bioRxiv : the preprint server for biology·2026
Same author

A genome-wide in vivo CRISPR screen identifies neuroprotective strategies in the mouse and human retina.

Neuron·2026

Related Experiment Video

Updated: May 2, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy
08:31

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

17.6K

Integrated photoacoustic, confocal, and two-photon microscope.

Bin Rao1, Florentina Soto2, Daniel Kerschensteiner2

  • 1Washington University in St. Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, One Brookings Drive, St. Louis, Missouri 63130.

Journal of Biomedical Optics
|March 5, 2014
PubMed
Summary

This study integrates optical-resolution photoacoustic microscopy with fluorescence microscopy, enabling label-free imaging of nonfluorescent molecules. This novel trimodality microscope advances biological and medical research by offering complementary contrast mechanisms.

More Related Videos

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
11:21

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

10.9K
Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging
10:17

Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging

Published on: June 26, 2017

11.4K

Related Experiment Videos

Last Updated: May 2, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy
08:31

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

17.6K
Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
11:21

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

10.9K
Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging
10:17

Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging

Published on: June 26, 2017

11.4K

Area of Science:

  • Biomedical Imaging
  • Optical Microscopy
  • Molecular Probes

Background:

  • Fluorescence microscopy, while powerful, is limited by the need for exogenous probes and inability to image nonfluorescent molecules.
  • Existing contrast agents can potentially alter cellular structures and functions, impacting experimental validity.

Purpose of the Study:

  • To overcome the limitations of fluorescence microscopy by integrating optical-resolution photoacoustic microscopy (OR-PAM).
  • To provide complementary, label-free optical absorption contrast alongside fluorescence imaging.
  • To establish a versatile trimodality microscope platform for diverse biological and medical applications.

Main Methods:

  • Integration of OR-PAM with a confocal, two-photon fluorescence microscope (Olympus IX81).
  • Development of automated coregistration for simultaneous imaging from the same sample.
  • Demonstration of imaging capabilities in ophthalmology, developmental biology, and plant science.

Main Results:

  • Successful integration of OR-PAM and fluorescence microscopy, creating a trimodality system.
  • Generation of automatically coregistered images with complementary contrast.
  • Demonstrated utility across multiple scientific disciplines, including ophthalmology and developmental biology.

Conclusions:

  • The developed trimodality microscope overcomes key limitations of traditional fluorescence microscopy.
  • This platform offers a novel approach for label-free imaging of nonfluorescent chromophores.
  • It provides a versatile tool for future biological and medical discoveries in a familiar microscopy setting.