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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Related Experiment Video

Updated: May 2, 2026

3' End Sequencing Library Preparation with A-seq2

3' End Sequencing Library Preparation with A-seq2

Published on: October 10, 2017

Quantitative polyadenylation site mapping with single-molecule direct RNA sequencing.

Fatih Ozsolak¹

¹Helicos BioSciences Corporation, One Kendall Square, Cambridge, MA, 02139, USA, fatihozsolak@gmail.com.

Methods in Molecular Biology (Clifton, N.J.)

|March 5, 2014

Summary

Understanding RNA polyadenylation is crucial for human health. New direct RNA sequencing methods enable precise mapping of polyadenylation sites, aiding disease research.

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Last Updated: May 2, 2026

3' End Sequencing Library Preparation with A-seq2

3' End Sequencing Library Preparation with A-seq2

Published on: October 10, 2017

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

Published on: December 2, 2009

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

Area of Science:

Molecular Biology
Genomics
Biochemistry

Background:

3' untranslated regions (3'UTRs) and poly(A) tails regulate RNA localization, stability, and translation.
Defects in polyadenylation are linked to human diseases like muscular dystrophy, thalassemias, thrombophilia, and IPEX syndrome.

Purpose of the Study:

To outline strategies for quantitative polyadenylation site mapping and analysis.
To leverage single-molecule direct RNA sequencing for comprehensive polyadenylation state characterization.

Main Methods:

Utilizing single-molecule direct RNA sequencing technology.
Developing quantitative methods for mapping and analyzing polyadenylation sites genome-wide.

Main Results:

Established strategies for precise polyadenylation site identification.
Enabled detailed analysis of polyadenylation states in various conditions.

Conclusions:

Characterizing genome-wide polyadenylation is essential for understanding normal physiology and disease.
Direct RNA sequencing offers a powerful tool for studying polyadenylation mechanisms and their role in human health.