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Producing and concentrating lenti-Cre for mouse infections
Jessica J Gierut1, Tyler E Jacks, Kevin M Haigis
1Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Department of Pathology, Harvard Medical School, Charlestown, Massachusetts 02129;
Cold Spring Harbor Protocols
|March 5, 2014
Abstract:
Lentiviral vectors offer versatility as vehicles for gene delivery. They can transduce a wide range of cell types and integrate into the host genome, which results in long-term expression of the transgene (Cre) both in vitro and in vivo. This protocol describes how lentiviral particles are produced, purified, and concentrated.
