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Related Experiment Videos

Development of an antigen-specific CD8 suppressor effector clone in man.

T Takeuchi1, S F Schlossman, C Morimoto

  • 1Division of Tumor Immunobiology, Dana-Farber Cancer Institute, Boston, MA.

Journal of Immunology (Baltimore, Md. : 1950)
|November 1, 1988
PubMed
Summary

A novel CD8 suppressor T cell clone (5B9) specifically inhibits keyhole limpet hemocyanin (KLH) antibody responses. This effector T cell clone

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Area of Science:

  • Immunology
  • T cell biology
  • Humoral immunity

Background:

  • CD8 T cells play diverse roles in immune regulation.
  • Suppressor T cells modulate immune responses, but their precise mechanisms require further elucidation.
  • Keyhole limpet hemocyanin (KLH) is a well-established antigen used in immunological studies.

Purpose of the Study:

  • To characterize a long-term cultured, KLH-specific CD8 suppressor T cell clone.
  • To determine the specificity and functional properties of this suppressor T cell clone.
  • To investigate the role of the CD3:TCR complex in suppressor T cell function.

Main Methods:

  • Generation of a KLH-specific CD8 suppressor T cell clone (5B9) from a hyperimmunized donor.
  • In vitro assessment of the clone's ability to suppress anti-KLH antibody production.

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  • Evaluation of cross-reactivity with other antigens (e.g., Tetanus Toxoid) and general immunoglobulin synthesis.
  • Analysis of the requirement for other immune cells (e.g., CD4+ cells) in the suppression assay.
  • Characterization of T cell receptor (TCR) expression and the impact of CD3 antigen modulation on suppressor function.

    • Main Results:

    • The 5B9 clone specifically suppressed anti-KLH antibody responses in vitro.
    • Suppression was KLH-specific, as anti-Tetanus Toxoid antibody production and PWM-driven IgG synthesis were unaffected.
    • The clone acted as an effector-type suppressor T cell, not requiring CD8+ cells for function when co-cultured with B cells and CD4+4B4+ cells.
    • Functional importance of the CD3:TCR complex was demonstrated, as its modulation abolished suppressor activity.

    Conclusions:

    • A KLH-specific CD8 suppressor T cell clone (5B9) has been characterized.
    • This clone represents an effector-type suppressor T cell whose function is dependent on the CD3:TCR complex.
    • The findings provide insights into the specific mechanisms of CD8-mediated immune suppression.