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Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

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Chiral nanostructures studied using polarization-dependent NOLES imaging.

Jeremy W Jarrett¹, Patrick J Herbert, Scott Dhuey

¹Department of Chemistry and Biochemistry, Florida State University , Tallahassee, Florida 32306-4390, United States.

The Journal of Physical Chemistry. A

|March 6, 2014

Summary

This summary is machine-generated.

The Nonlinear Optical Localization using Electromagnetic Surface fields (NOLES) imaging technique precisely locates chiral objects using asymmetric gold nanostructures. This method achieves nanometer precision by analyzing second-harmonic generation, offering a powerful tool for chiral specimen study.

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Spectral and Angle-Resolved Magneto-Optical Characterization of Photonic Nanostructures

Spectral and Angle-Resolved Magneto-Optical Characterization of Photonic Nanostructures

Published on: November 21, 2019

Area of Science:

Nanophotonics
Chirality
Optical Imaging

Background:

Chiral objects exhibit asymmetry, crucial in fields like chemistry and biology.
Precisely determining the position of chiral nanostructures is challenging.
Existing imaging techniques may lack the required spatial resolution for nanoscale chiral analysis.

Purpose of the Study:

To demonstrate the capability of the Nonlinear Optical Localization using Electromagnetic Surface fields (NOLES) imaging technique for high-precision localization of chiral objects.
To investigate the role of asymmetric nanostructures in enhancing chiral imaging contrast and localization accuracy.
To establish a correlation between the magnetic dipole to electric dipole ratio and imaging performance.

Main Methods:

Utilized asymmetric gold bowtie nanostructures as a model system with 2D chirality.
Employed a Ti:sapphire laser to generate second-harmonic frequency from the nanostructures.
Performed single-particle second-harmonic generation circular dichroism (SHG-CDR) and continuous polarization variation (CPV) measurements.
Validated results using finite difference time domain (FDTD) simulations.
Analyzed image point spread functions for spatial localization precision.

Main Results:

Achieved nanometer precision in determining the position of chiral objects.
Demonstrated that chiral image contrast and localization precision depend on the magnetic dipole/electric dipole ratio (G/F).
Obtained a localization precision of 1.13 ± 0.13 nm with 400% image enhancement for bowties with high G/F ratios.
Confirmed polarization dependence and magnetic dipole amplification effects in NOLES imaging.

Conclusions:

The NOLES technique offers a powerful method for studying chiral specimens with exceptional spatial precision.
Asymmetric nanostructures, particularly those with high G/F ratios, significantly enhance imaging performance.
NOLES imaging provides a valuable tool for nanoscale characterization of chiral materials and phenomena.