Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Extracellular matrix alterations during endochondral ossification in humans.

W A Horton1, M M Machado

  • 1Department of Pediatrics, University of Texas Medical School, Houston.

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
|January 1, 1988
PubMed
Summary

During human endochondral ossification, cartilage extracellular matrix changes. Proteoglycans and collagen type II decrease in differentiating cell zones, facilitating vascular invasion, while septa remain intact.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fluconazole induces genotoxicity in cultured human peripheral blood mononuclear cells via immunomodulation of TNF-α, IL-6, and IL-10: new challenges for safe therapeutic regimens.

Immunopharmacology and immunotoxicology·2019
Same author

Anti-dermatophyte activity of Leguminosae plants from Southern Brazil with emphasis on Mimosa pigra (Leguminosae).

Journal de mycologie medicale·2017
Same author

Biodiesel production using fatty acids from food industry waste using corona discharge plasma technology.

Waste management (New York, N.Y.)·2015
Same author

Imidazolium salts with antifungal potential against multidrug-resistant dermatophytes.

Journal of applied microbiology·2015
Same author

Analysis of cellular adhesion on superhydrophobic and superhydrophilic vertically aligned carbon nanotube scaffolds.

Materials science & engineering. C, Materials for biological applications·2015
Same author

Human proximal femur bone adaptation to variations in hip geometry.

Bone·2014

Area of Science:

  • Biochemistry
  • Cell Biology
  • Histology

Background:

  • Endochondral ossification is a complex biological process crucial for skeletal development.
  • The cartilage extracellular matrix (ECM) undergoes significant remodeling during this process.
  • Understanding ECM changes is vital for comprehending bone formation and potential developmental abnormalities.

Purpose of the Study:

  • To investigate the dynamic alterations in cartilage ECM components during human endochondral ossification.
  • To map the distribution of key ECM molecules, including proteoglycans and collagens, in the growth plate.

Main Methods:

  • Immunohistochemical analysis using the avidin-biotin-horseradish peroxidase system.
  • Examination of human iliac crest growth-plate specimens.

Related Experiment Videos

  • Detection of chondroitin sulfate isomers, keratan sulfate, proteoglycan (PG) link protein, collagen types I and II, and fibronectin.

    • Main Results:

    • Collagen type II forms a structural framework throughout the growth plate.
    • Proteoglycans, PG link protein, and collagen type II are abundant in inter-cluster septa, resembling reserve cartilage.
    • Differentiating cell clusters show reduced collagen type II, PG link protein, and cartilage PG.
    • Collagen type I and fibronectin are found in maturing/degenerating cell cytoplasm and pericellular envelopes.

    Conclusions:

    • ECM alterations in differentiating zones facilitate vascular invasion and matrix degradation.
    • Intact septa, rich in characteristic cartilage matrix components, anchor the growth plate to subchondral bone.
    • These findings elucidate the ECM's role in regulating the orchestrated process of endochondral ossification.