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Updated: May 2, 2026

A Quantitative Glycomics and Proteomics Combined Purification Strategy

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Published on: March 8, 2016

O-GlcNAc profiling: from proteins to proteomes.

Junfeng Ma, Gerald W Hart¹

¹Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205-2185, USA. gwhart@jhmi.edu.

Clinical Proteomics

|March 6, 2014

Summary

O-linked N-acetylglucosamine (O-GlcNAc) is a key protein modification impacting many biological processes. Technological advancements in O-GlcNAc profiling are crucial for understanding its role in chronic diseases like diabetes and cancer.

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Area of Science:

Biochemistry
Molecular Biology
Cellular Biology

Background:

O-linked β-D-N-acetylglucosamine (O-GlcNAc) modification (O-GlcNAcylation) is a critical post-translational modification (PTM) regulating numerous cellular functions.
Dysregulated O-GlcNAcylation is implicated in the pathogenesis of chronic diseases, including cancer, diabetes, and neurodegenerative disorders.

Purpose of the Study:

To review technological advancements in O-GlcNAc protein profiling.
To highlight the application of these techniques for characterizing targeted proteins and enabling systems-level O-GlcNAcomic profiling.
To discuss emerging trends for understanding the physiological and pathological roles of O-GlcNAcylation.

Main Methods:

Review of current literature on O-GlcNAc protein profiling technologies.
Analysis of methods for targeted protein characterization and global O-GlcNAcomic profiling.
Discussion of systems biology approaches to study PTM crosstalk.

Main Results:

Technological developments enable comprehensive characterization of O-GlcNAc modified proteins.
Newly developed approaches facilitate O-GlcNAcomic profiling, offering a systems perspective on biological events.
These advancements are vital for deciphering the functions of O-GlcNAc proteins.

Conclusions:

Advancements in O-GlcNAc profiling technologies are essential for understanding protein O-GlcNAcylation.
These techniques provide insights into the complex roles of O-GlcNAcylation in health and disease.
Further research into promising technical trends will deepen our understanding of O-GlcNAcylation's involvement in chronic diseases.