Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microtubule Instability02:17

Microtubule Instability

5.0K
Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated...
5.0K
Microtubule Instability02:17

Microtubule Instability

5.2K
5.2K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

10.2K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
10.2K
Destabilization of Microtubules01:45

Destabilization of Microtubules

2.9K
The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...
2.9K
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

4.9K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
4.9K
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

3.1K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
3.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MYH11 variants in thoracic aortic aneurysm pathophysiology: From bench to bedside.

European journal of clinical investigation·2026
Same author

Micropatterning transcriptionally and phenotypically reprograms endothelial cells.

Acta biomaterialia·2026
Same author

In vivo endosomal escape assay identifies mechanisms for efficient hepatic LNP delivery.

Nature biotechnology·2026
Same author

Evolutionary remodeling of the FXR binding pocket drove functional divergence and ligand discrimination.

Molecular biology and evolution·2026
Same author

Conformational Dynamics and Catalytic Backups in a Hyper-thermostable Engineered Archaeal Protein Tyrosine Phosphatase.

JACS Au·2026
Same author

Long-term efficacy of migalastat in females with Fabry disease.

Journal of medical genetics·2025

Related Experiment Video

Updated: May 2, 2026

In vivo Assessment of Microtubule Dynamics and Orientation in Caenorhabditis elegans Neurons
07:43

In vivo Assessment of Microtubule Dynamics and Orientation in Caenorhabditis elegans Neurons

Published on: November 20, 2021

2.6K

STRAD pseudokinases regulate axogenesis and LKB1 stability.

Biliana O Veleva-Rotse, James L Smart, Annette F Baas

  • 1Department of Pediatrics-Doernbecher, Children's Hospital, Portland, OR 97239, USA. barnesan@ohsu.edu.

Neural Development
|March 6, 2014
PubMed
Summary

STRADα and STRADβ are crucial for brain development, with STRADα uniquely stabilizing LKB1 protein levels. Both STRAD paralogs promote neuron survival and axon formation.

More Related Videos

High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells
10:25

High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells

Published on: August 13, 2016

13.7K
Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
08:13

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

799

Related Experiment Videos

Last Updated: May 2, 2026

In vivo Assessment of Microtubule Dynamics and Orientation in Caenorhabditis elegans Neurons
07:43

In vivo Assessment of Microtubule Dynamics and Orientation in Caenorhabditis elegans Neurons

Published on: November 20, 2021

2.6K
High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells
10:25

High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells

Published on: August 13, 2016

13.7K
Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
08:13

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

799

Area of Science:

  • Neuroscience
  • Cell Biology
  • Developmental Biology

Background:

  • Neuronal polarization is vital for brain development and connectivity.
  • LKB1, a kinase regulating cell polarity and axon formation, is controlled by STRADα and STRADβ pseudokinases.
  • The specific roles of STRADα and STRADβ in neural development are not fully understood.

Purpose of the Study:

  • To investigate the distinct functions of STRADα and STRADβ in the developing nervous system.
  • To elucidate the regulatory relationship between STRAD paralogs and LKB1 in axogenesis.

Main Methods:

  • Analysis of STRADα splicing.
  • In vivo studies in the developing cortex to assess STRAD functions.
  • Investigation of protein-stabilizing interactions between LKB1 and STRADs.

Main Results:

  • STRADα is highly spliced and appears to be the ancestral paralog.
  • Both STRADα and STRADβ support axogenesis and cell survival in the developing cortex.
  • STRADα maintains LKB1 protein stability in vivo through cytoplasmic localization.

Conclusions:

  • STRADβ plays a novel role in axogenesis.
  • STRADα, but not STRADβ, is essential for maintaining LKB1 protein stability in vivo.
  • These findings clarify the distinct contributions of STRAD paralogs to neuronal development.