Circulating endothelial progenitor cells as markers for severity of ischemic chronic heart failure
Alexander E Berezin1, Alexander A Kremzer2
1Internal Medicine Department, State Medical University, Zaporozhye, Ukraine.
Insights
Endothelial progenitor cell (EPC) counts are altered in chronic heart failure (CHF). Specific EPC subpopulations correlate with heart dysfunction and coronary artery disease severity, offering diagnostic potential.
Area of Science:
- Cardiovascular Research
- Cell Biology
- Regenerative Medicine
Background:
- Endothelial progenitor cells (EPCs) show diagnostic potential, but their role in ischemic chronic heart failure (CHF) remains unclear.
- Investigating specific circulating EPC subpopulations is crucial for understanding their involvement in CHF pathogenesis.
Purpose of the Study:
- To assess counts of specific phenotyped circulating EPC subpopulations (CD45(+)CD34(+), CD45(-)CD34(+), CD14(+)CD309(+), CD14(+)CD309(+)Tie2(+)) in patients with ischemic CHF.
- To determine the association between EPC counts and the severity of CHF and coronary artery disease (CAD).
Main Methods:
- Phenotyping of mononuclear cell populations using flow cytofluorimetry in 153 CAD patients and 25 healthy volunteers.
- Assessment of cardiovascular risk factors and their impact on circulating EPC counts.
- Correlation analysis between EPC subpopulations and clinical parameters of CHF and CAD severity.
Main Results:
- Cardiovascular risk factors negatively affect EPC counts in CAD patients, irrespective of CHF status.
- Depletion of CD14(+)CD309(+) and CD14(+)CD309(+)Tie2(+) EPCs correlates with left ventricular dysfunction severity.
- CD45(+)CD34(+) and CD45(-)CD34(+) cell counts reflect the severity of atherosclerotic coronary artery lesions.
Conclusions:
- New York Heart Association functional class, low left ventricular ejection fraction, elevated N-terminal pro-B-type natriuretic peptide, and high E/Em ratio predict reduced EPC counts in CAD patients.
- Specific EPC subpopulations serve as valuable biomarkers for assessing CHF severity and CAD progression.
Introduction:
Despite a high potential of endothelial progenitor cells (EPCs) for diagnostic purposes, the EPC role in developing ischemic chronic heart failure (CHF) has not been determined obviously. The objective of this study was to assess the counts of CD45(+)CD34(+), CD45(-)CD34(+), CD14(+)CD309(+), and CD14(+)CD309(+)Tie2(+) phenotyped circulating EPCs of various subpopulations in patients with ischemic CHF.
Methods And Results:
The study involved 153 patients (86 male), aged 48-62 years, with angiographically proven coronary artery disease (CAD) and 25 healthy volunteers. CHF was diagnosed in 109 patients (71.2%). Mononuclear cell populations were phenotyped by flow cytofluorimetry. Cardiovascular risk factors, such as type 2 diabetes mellitus, hyperlipidemia, arterial hypertension, and adherence to smoking, may have a negative effect on circulating EPC counts in CAD patients regardless of the presence of CHF. The depletion of the CD14(+)CD309(+)- and CD14(+)CD309(+)Tie2(+)-phenotyped circulating EPC counts is associated with the severity of left ventricular dysfunction, whereas the CD45(+)CD34(+)- and CD45(-)CD34(+)-mononuclear cell counts are more representative of the severity of atherosclerotic coronary artery lesions.
Conclusion:
The authors found that New York Heart Association functional class of CHF, left ventricular ejection fraction <42%, the N-terminal pro-B-type natriuretic peptide level >554 pg/mL, and Е/Еm ratio >15 U had the highest predictive value for the depletion of the EPC count in CAD patients.
More Related Videos
07:25Predicting Amputation using Local Circulating Mononuclear Progenitor Cells in Angioplasty-treated Patients with Critical Limb Ischemia
Published on: September 22, 2020
10:03Coronary Progenitor Cells and Soluble Biomarkers in Cardiovascular Prognosis after Coronary Angioplasty
Published on: January 28, 2020
