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Updated: May 2, 2026
A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks
Published on: June 26, 2020
Detection and localization of rabbit hepatitis e virus and antigen in systemic tissues from experimentally
Jingjing Mao1, Yue Zhao1, Ruiping She1
1Department of Veterinary Pathology, Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Key Laboratory of Zoonosis of Ministry of Agriculture, Beijing, China.
Abstract:
Rabbit hepatitis E virus (HEV) is a novel genotype of HEV, and is considered to pose a risk of zoonotic transmission. Research into the systemic distribution of rabbit HEV in rabbits during different periods of infection has rarely been reported. To better understand this virus, we infected rabbits with second-passage rabbit HEV via an intraperitoneal route. After inoculation, the infection showed two types, temporary and constant infection. The detection of HEV RNA in the feces varied with time, and serum antigen correlated with fecal HEV RNA. Viremia only appeared 72 days after inoculation. The rabbits remained antibody negative throughout the experimental period. When HEV was localized, several organs besides the liver were HEV RNA positive. Tissue antigen was observed immunohistochemically in the different cells of various organs, especially in parts of the small intestine and the characteristic rabbit gut-associated lymphoid tissue. These data provide valuable information for future research into the pathogenesis of HEV.
