Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

2.0K
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
2.0K
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

3.0K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
3.0K
High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

2.9K
High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
2.9K
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

3.0K
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
3.0K
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

2.8K
In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
2.8K
Gas Chromatography: Types of Columns and Stationary Phases01:17

Gas Chromatography: Types of Columns and Stationary Phases

4.1K
Gas chromatography (GC) relies on stationary phases to separate and analyze components in a sample. There are two main types of stationary phases: liquid and solid. Liquid stationary phases are non-volatile, thermally stable, and chemically inert liquids coated onto the column. Solid stationary phases are particles of adsorbent material, such as silica gel or molecular sieves.
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
4.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Collection and identification of an unknown component from Eugenia uniflora essential oil exploiting a multidimensional preparative three-GC system employing apolar, mid-polar and ionic liquid stationary phases.

Faraday discussions·2019
Same author

Variation of anionic moieties of dicationic ionic liquid GC stationary phases: Effect on stability and selectivity.

Analytica chimica acta·2018
Same author

Gas chromatography selectivity of new phosphonium-based dicationic ionic liquid stationary phases.

Journal of separation science·2018
Same author

Correction to: Branched-chain dicationic ionic liquids for fatty acid methyl ester assessment by gas chromatography.

Analytical and bioanalytical chemistry·2018
Same author

Branched-chain dicationic ionic liquids for fatty acid methyl ester assessment by gas chromatography.

Analytical and bioanalytical chemistry·2017
Same author

Retention behavior of isomeric polycyclic aromatic sulfur heterocycles in gas chromatography on stationary phases of different selectivity.

Journal of chromatography. A·2017

Related Experiment Video

Updated: May 2, 2026

Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization
10:41

Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization

Published on: April 5, 2019

18.5K

A new interface for coupling solid phase microextraction with liquid chromatography.

Yong Chen1, Leonard M Sidisky1

  • 1Supelco, 595 North Harrison Road, Bellefonte, PA 16823, USA.

Analytica Chimica Acta
|March 6, 2014
PubMed
Summary
This summary is machine-generated.

A novel solid phase microextraction (SPME)-liquid chromatography (LC) interface was developed using a modified microsample injector. This new interface efficiently analyzes polycyclic aromatic hydrocarbons (PAHs) in water with high sensitivity and repeatability.

Keywords:
InterfaceLiquid chromatography (LC)Polycyclic aromatic hydrocarbons (PAHs)Solid phase microextraction (SPME)

More Related Videos

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
07:55

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells

Published on: June 21, 2019

6.1K
Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples
09:42

Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples

Published on: August 7, 2016

11.3K

Related Experiment Videos

Last Updated: May 2, 2026

Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization
10:41

Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization

Published on: April 5, 2019

18.5K
Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
07:55

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells

Published on: June 21, 2019

6.1K
Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples
09:42

Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples

Published on: August 7, 2016

11.3K

Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Solid Phase Microextraction (SPME) is a widely used technique for sample preparation.
  • Coupling SPME directly with Liquid Chromatography (LC) can enhance analytical efficiency.
  • Existing SPME-LC interfaces face challenges in solvent delivery and carryover.

Purpose of the Study:

  • To develop a new, reliable interface for coupling SPME with LC.
  • To evaluate the analytical performance of the developed SPME-LC interface.
  • To analyze polycyclic aromatic hydrocarbons (PAHs) in water samples.

Main Methods:

  • Modification of a Rheodyne 7520 microsample injector to create a new sample rotor.
  • Enlargement of the central flow passage to serve as a static desorption chamber.
  • SPME-LC analysis of PAHs using a polyacrylonitrile (PAN)/C18 bonded fuse silica fiber.

Main Results:

  • High desorption efficiency (>90%) of PAHs achieved within 30 seconds.
  • Rapid injection completed within 20 seconds.
  • Repeatability ranged from 2% to 8%, with limits of detection in the mid pg mL(-1) range.
  • Carryover in the interface was minimized by flushing with acetonitrile.
  • Linear ranges for analysis were established from 0.1 to 100 ng mL(-1).

Conclusions:

  • The modified microsample injector serves as a reliable SPME-LC interface.
  • The interface enables efficient qualitative and quantitative analysis of PAHs.
  • This development offers a robust solution for integrating SPME with LC systems.