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Microbial Bioremediation of Pesticides01:28

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Pesticides often feature structurally complex chemical architectures, incorporating halogen groups and multiple aromatic rings. These characteristics confer high chemical stability, rendering many pesticides resistant to natural degradation processes. This resistance poses significant environmental concerns, as persistent pesticide residues can accumulate in ecosystems and affect non-target organisms.Despite the inherent stability of many pesticides, certain microorganisms possess the metabolic...
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Trypsin-catalyzed deltamethrin degradation.

Chunrong Xiong1, Fujin Fang1, Lin Chen1

  • 1Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, China; Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, China.

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This study shows that trypsin can degrade the pesticide deltamethrin, significantly reducing its toxicity. This finding suggests trypsin

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Area of Science:

  • Biochemistry
  • Environmental Science
  • Toxicology

Background:

  • Pesticides like deltamethrin pose environmental and health risks.
  • Proteases, such as trypsin, are enzymes typically known for protein degradation.
  • The potential of proteases to degrade non-protein molecules remains underexplored.

Purpose of the Study:

  • To investigate whether trypsin can catalyze the degradation of the non-protein molecule deltamethrin.
  • To characterize the degradation products of deltamethrin by trypsin.
  • To assess the acute oral toxicity of deltamethrin and its degradation products.

Main Methods:

  • In vitro hydrolytic reactions of deltamethrin with and without trypsin.
  • Analysis using ultraviolet-visible (UV/Vis) spectrophotometry.
  • Analysis using gas chromatography-mass spectrometry (GC/MS).
  • Acute oral toxicity testing (LD50) in Wistar rats.

Main Results:

  • Spectrophotometry and GC/MS confirmed deltamethrin degradation by trypsin, indicated by shifts in absorption peaks and retention times.
  • The LD50 of deltamethrin was 55 mg/kg.
  • The LD50 of deltamethrin degradation products was significantly higher (3358 mg/kg in females, 1045 mg/kg in males), indicating a substantial reduction in toxicity.
  • Trypsin directly degraded deltamethrin, reducing its toxicity by up to 61-fold.

Conclusions:

  • Trypsin effectively catalyzes the degradation of the pesticide deltamethrin.
  • The degradation process significantly reduces the toxicity of deltamethrin.
  • Trypsin shows potential as an environmentally friendly agent for controlling pesticide residues on agricultural products and in the environment.