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Published on: May 24, 2020
Clinical CVVH model removes endothelium-derived microparticles from circulation
Abdelhafeez H Abdelhafeez1, Paul M Jeziorczak2, Terry R Schaid2
1Children's Research Institute, Milwaukee, WI, USA ; Division of Pediatric Surgery, Department of Surgery, Medical College of Wisconsin, Milwaukee, WI, USA ; Clinical and Translational Science Institute of Southeast Wisconsin, Milwaukee, WI, USA.
Continuous venovenous hemofiltration (CVVH) effectively reduces circulating endothelium-derived microparticles (EMPs) in a model system. This suggests CVVH may offer a novel therapeutic approach for acute lung injury (ALI) by lowering EMP levels.
Area of Science:
- Cardiovascular Biology
- Pulmonary Medicine
- Biomedical Engineering
Background:
- Endothelium-derived microparticles (EMPs) are implicated in acute lung injury (ALI) and endothelial barrier dysfunction.
- Current ALI treatments are limited to supportive care.
- Continuous venovenous hemofiltration (CVVH) is a potential therapeutic intervention to reduce EMPs.
Purpose of the Study:
- To investigate the efficacy of standard clinical CVVH in reducing circulating EMP levels.
- To evaluate the potential of CVVH as a therapeutic strategy for ALI.
Main Methods:
- EMPs were generated from activated human umbilical vein endothelial cells (HUVECs).
- Flow cytometry was used to characterize and enumerate EMPs.
- A standard CVVH circuit with a 200 μm pore filter was used to process a 470 mL volume of perfusate containing EMPs at a flow rate of 250 mL/hr for 70 minutes.
Main Results:
- Standard CVVH demonstrated a progressive decrease in circulating EMP concentration.
- A 50% reduction in EMPs was observed within the first 30 minutes of CVVH treatment.
- After 4 hours, only 5.7% of the original EMP concentration was found in the dialysate.
Conclusions:
- Standard CVVH effectively removes EMPs from circulation in a patient-模拟 circuit.
- Further investigation using an animal model is necessary to confirm the therapeutic potential of CVVH in ALI treatment.
