Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Overview of Regeneration and Repair

Overview of Regeneration and Repair

Regeneration and repair processes are critical in healing damages caused by injury, disease, and aging. In regeneration, the damaged tissue is entirely replaced with new growth that restores the original architecture and function. In contrast, tissue repair usually results in a fixed tissue architecture involving scar formation. Scars generally do not reestablish tissue function and may also exhibit structural abnormalities at the injury site.
Regeneration
All animals have varying degrees of...

Whole Body Regeneration

Whole Body Regeneration

Regeneration is the process of restoring injured or lost tissues, organs, or body parts. While simpler organisms generally show greater ability to regenerate their whole body, few complex animals show similarly exceptional regeneration. For example, planarian flatworms have a unique regenerative potential making them a popular study organism among biologists to understand the mechanisms of whole body regeneration. Other organisms, such as hydra, also show extreme regeneration potential;...

Cell Culture

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

<i>In Vitro</i> Coculture of Primary Human Cells to Analyze Angiogenesis, Osteogenesis, and the Inflammatory Response to Newly Developed Osteosynthesis Material for Pediatric Maxillofacial Traumatology: A Potential Pretesting Model before <i>In Vivo</i> Experiments.

Journal of tissue engineering and regenerative medicine·2025

Same author

In Vivo Efficacy of Neutrophil-Mediated Bone Regeneration Using a Rabbit Calvarial Defect Model.

International journal of molecular sciences·2021

Same author

Covalent linkage of sulfated hyaluronan to the collagen scaffold Mucograft® enhances scaffold stability and reduces proinflammatory macrophage activation <i>in vivo</i>.

Bioactive materials·2021

Same author

Multinucleated Giant Cells Induced by a Silk Fibroin Construct Express Proinflammatory Agents: An Immunohistological Study.

Materials (Basel, Switzerland)·2021

Same author

Thermal treatment at 500 °C significantly reduces the reaction to irregular tricalcium phosphate granules as foreign bodies: An in vivo study.

Acta biomaterialia·2020

Same author

Improved detection of sentinel lymph node metastases allows reliable intraoperative identification of patients with extended axillary lymph node involvement in early breast cancer.

Clinical & experimental metastasis·2020

Same journal

A Novel 3D Bioprinting Strategy for Bioengineering of Urethra with Clinical Relevance.

Tissue engineering. Part A·2026

Same journal

Hydrogel-Encapsulated Primed MSCs Enhance Regeneration in Full-Thickness Porcine Burn Wounds.

Tissue engineering. Part A·2026

Same journal

Unidirectional Porous Carbonate Apatite Fabricated by Gelatin-Based Freeze Casting for Bone Regeneration.

Tissue engineering. Part A·2026

Same journal

Regenerative Nanoscaffolds for Chronic Tympanic Membrane Perforation: From Bench to Clinical Translation.

Tissue engineering. Part A·2026

Same journal

Impact of IFN-γ-Pretreated Umbilical Cord Mesenchymal Stem Cells Implanted in Mesh on Pelvic Organ Prolapse.

Tissue engineering. Part A·2026

Same journal

The Driving Force of Hierarchical Collagen Fiber Formation: A Review of Tendon, Ligament, and Meniscus Mechanobiology.

Tissue engineering. Part A·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 2, 2026

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

Developing cellular systems in vitro to simulate regeneration.

Charles James Kirkpatrick¹

¹1 REPAIR-Lab, Institute of Pathology, University Medical Center, Johannes Gutenberg University , Mainz, Germany .

Tissue Engineering. Part A

|March 7, 2014

Summary

This summary is machine-generated.

Advancing biomaterial research, scientists are moving from single-cell to coculture systems for tissue regeneration. Future models will incorporate physiological stem cell niches, hypoxia, and inflammation for better in vitro studies.

More Related Videos

Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity

Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity

Published on: January 30, 2021

Adapting the Electrospinning Process to Provide Three Unique Environments for a Tri-layered In Vitro Model of the Airway Wall

Adapting the Electrospinning Process to Provide Three Unique Environments for a Tri-layered In Vitro Model of the Airway Wall

Published on: July 31, 2015

Related Experiment Videos

Last Updated: May 2, 2026

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity

Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity

Published on: January 30, 2021

Adapting the Electrospinning Process to Provide Three Unique Environments for a Tri-layered In Vitro Model of the Airway Wall

Adapting the Electrospinning Process to Provide Three Unique Environments for a Tri-layered In Vitro Model of the Airway Wall

Published on: July 31, 2015

Area of Science:

Biomaterials Science
Regenerative Medicine
Cellular Biology

Background:

In vitro models have evolved from monocellular to coculture systems for biomaterial testing.
Understanding cellular cross talk is crucial for bone vascularization and tissue regeneration.

Purpose of the Study:

To review the progression of in vitro cellular systems for biomaterial evaluation and regeneration.
To highlight future challenges and opportunities in developing advanced coculture models.

Main Methods:

Review of advancements in cellular systems, from monocellular to coculture models.
Discussion of incorporating physiological stem cell niches, hypoxia, and inflammatory status.
Highlighting the role of polymer synthesis in creating functional hydrogels.

Main Results:

Significant progress in understanding cellular cross talk for bone vascularization.
Development of coculture systems combining progenitor cells and novel biomaterials.
Advances in polymer synthesis enable the incorporation of biological signals into hydrogels.

Conclusions:

Future in vitro models should integrate physiological stem cell niches and dynamic factors like hypoxia and inflammation.
Novel biomaterials with incorporated signals in hydrogels are key for in situ regenerative niche investigation.
Coculture systems represent a significant step towards more relevant in vitro models for regenerative medicine.