[Prospective study on comparing two methods: for dynamic monitoring of minimal residual disease in acute
Yan Zhang1, Li-Ping Tian1, Ying Zhang1
1Department of Hematology, Beijing Longfu Hospital, 100010 Beijing, China.
Abstract:
This study was purposed to comparatively analyse the value of PCR and FCM for dynamic monitoring minimal residual disease (MRD) of acute promyelocytic leukemia. The patients with acute promyelocytic leukemia hospitalized in our hospital from January 2011 to December 2012 were observed and all achieved complete remission after remission induction therapy. Before the chemotherapy, the bone marrow cell morphology examination, polymerase-chain reaction (PCR) and multi-parameter flow cytometry (FCM) were performed for each patient. Then the detection results were statistically analyzed. The 477 specimens were achieved from 159 detections for 48 patients. The results showed that 3 specimens were found to be relapsed by bone marrow cell examination, and other specimens were complete remission;PCR detection confirmed 7 positive, and the FCM confirmed 19 positive. There wasn't significant difference between PCR and FCM by kappa test (P > 0.05). It is concluded that FCM is as sensitive as PCR in evaluating the treatment effect of acute promyelocytic leukemia.
Insights
Flow cytometry (FCM) and polymerase-chain reaction (PCR) are equally effective for monitoring minimal residual disease (MRD) in acute promyelocytic leukemia patients. Both methods accurately assess treatment response in patients achieving complete remission.
Area of Science:
- Hematology
- Oncology
- Molecular Diagnostics
Background:
- Acute promyelocytic leukemia (APL) requires effective monitoring for minimal residual disease (MRD).
- Assessing treatment efficacy is crucial for managing APL patients post-remission induction therapy.
Purpose of the Study:
- To comparatively analyze the diagnostic value of Polymerase-Chain Reaction (PCR) and Multi-parameter Flow Cytometry (FCM) for dynamic MRD monitoring in APL.
- To evaluate the sensitivity of PCR and FCM in detecting residual disease after treatment.
Main Methods:
- Bone marrow samples from 48 APL patients in complete remission were analyzed.
- Techniques included bone marrow cell morphology, PCR, and multi-parameter FCM.
- Statistical analysis, including kappa test, was used to compare PCR and FCM results.
Main Results:
- Out of 477 specimens, bone marrow morphology indicated relapse in 3 cases; all others showed complete remission.
- PCR detected 7 positive cases, while FCM identified 19 positive cases.
- Kappa test showed no significant difference between PCR and FCM (P > 0.05).
Conclusions:
- FCM demonstrates comparable sensitivity to PCR in evaluating treatment effectiveness for APL.
- Both PCR and FCM are valuable tools for the dynamic monitoring of MRD in APL patients.
- FCM offers a sensitive alternative for assessing treatment response in APL.
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