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[Distribution of compact bone mesenchymal stem cells in lung tissue and bone marrow of mouse]
Rui-Ping Wang1, Ren-Na Wu2, Yu-Qing Guo2
1Department of Hematopoietic Stem Cell Transplantation, Chinese PLA Hospital 307, Beijing 100071, China; Department of Hematology, Inner Mongolia Medical University Chifeng Municipal Hospital, Chifeng 024000, Inner Mongolian Autonomous Region, China.
Abstract:
This study was aimed to investigate the distribution of compact bone mesenchymal stem cells(MSC) marked with lentiviral plasmid pGC FU-RFP-LV in lung tissue and bone marrow of mouse. The MSC were infected by lentivirus with infection efficiency 78%, the infected MSC were injected into BALB/c mice via tail veins in concentration of 1×10(6) /mouse. The mice were randomly divided into 4 group according to 4 time points as 1, 2, 5 and 7 days. The lung tissue and bone marrow were taken and made of frozen sections and smears respectively in order to observed the distributions of MSC. The results indicated that the lentiviral infected MSC displayed phenotypes and biological characteristics which conformed to MSC by immunophenotyping analysis and induction differentiation detection. After the MSC were infected with optimal viral titer MOI = 50, the cell growth no significantly changed; the fluorescent microscopy revealed that the distributions of MSC in bone marrow on day 1, 2, 5 and 7 were 0.50 ± 0.20, 0.67 ± 0.23, 0.53 ± 0.14, 0.33 ± 0.16; those in lung tissue were 0.55 ± 0.15, 0.47 ± 0.13, 0.29 ± 0.13, 0.26 ± 0.08. It is concluded that the distribution of MSC in lung tissue reaches a peak on day 1, while distribution of MSC in bone marrow reaches a peak on day 2. The distribution of mouse MSC relates with RFP gene expression and implantation of MSC in lung tissue and bone marrow.
Insights
Mesenchymal stem cells (MSC) distribution in mouse lungs and bone marrow was studied after tail vein injection. MSCs peaked in lung tissue on day 1 and bone marrow on day 2, indicating distinct homing patterns.
Area of Science:
- Stem Cell Biology
- Regenerative Medicine
- Mouse Models
Background:
- Mesenchymal stem cells (MSCs) are crucial for tissue repair and regeneration.
- Understanding MSC distribution is vital for optimizing cell-based therapies.
- Lentiviral vectors offer efficient gene delivery for tracking cell behavior.
Purpose of the Study:
- To investigate the biodistribution and homing patterns of lentivirally-labeled compact bone MSCs in mice.
- To determine the temporal distribution of MSCs in lung tissue and bone marrow.
- To assess the impact of lentiviral labeling on MSC characteristics.
Main Methods:
- Compact bone MSCs were labeled with a lentiviral vector expressing RFP.
- Infected MSCs (78% efficiency) were injected intravenously into BALB/c mice.
- Mice were analyzed at 1, 2, 5, and 7 days post-injection.
- Frozen sections of lung tissue and bone marrow smears were examined using fluorescent microscopy.
Main Results:
- Lentiviral-infected MSCs retained their characteristic phenotypes and differentiation potential.
- Optimal viral titer (MOI=50) did not significantly alter MSC growth.
- MSCs were detected in bone marrow (peak day 2) and lung tissue (peak day 1).
- Distribution patterns correlated with RFP gene expression and MSC implantation.
Conclusions:
- Mouse MSC distribution exhibits distinct temporal dynamics in lung and bone marrow following intravenous injection.
- Lung tissue shows an early peak distribution of MSCs, while bone marrow shows a slightly later peak.
- Lentiviral labeling is a viable method for tracking MSCs, with observed distribution patterns linked to RFP expression.
