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Updated: May 2, 2026
Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
Published on: March 8, 2012
Research on sequence variations analysis of HPV-16 type in Southwestern China
1Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, Institute of Medical Genetics, College of Life Sciences, Sichuan University, Chengdu, China.
Objective:
This study was designed to analyze sequence variations in E6, E7, and L1 of human papillomavirus type 16 (HPV-16) to identify novel HPV-6 variants and correlate them with the progression of cervical cancer.
Materials And Methods:
Cervical biopsies from 12 HPV-16 positive cervical neoplasia cases were analyzed by polymerase chain reaction (PCR) for E6,E7, and L1 gene. The products of PCR were ligated into pGEM-T vector and used to transform e. coli DH5a. Finally, the sequence of the insert was determined by an automated DNA sequencer.
Results:
Compared with the European-Germanyl31 (EG131), 20 mutations were detected, of which eight mutations were detected from all the four biopsies: 131(G-A)(Gly-Arg), 178 (T-G)(Asp-Glu),350 (G-T)(Val-Leu), 647 (A-G)(Asn-Asp),846 (T-C) (synonymous mutation), L1 966th (C-T)(synonymous mutation),L1 1302 (C-T)(synonymous mutation) and L1 1434th (A-G) (synonymous mutation).
Conclusions:
Compared with other HPV-16 reference sequences, nucleotide sequencing analysis of the HPV-16, E6,E7, and L1 gene from cervical carcinoma biopses in Southwestern China consistently shows point mutations, including synonymous mutation and nonsynonymous mutation. Above all, the results may have implications for future researches of viral persistence, transmission, oncogenic potential, developing diagnostic probe, and designing vaccine or biological preparation for a particular population.
