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Proline-rich protein is a glycoprotein and an acute phase reactant
M Funakoshi1, J Sasaki, K Arakawa
1Department of Internal Medicine, School of Medicine, Fukuoka University, Japan.
Biochimica Et Biophysica Acta
|November 4, 1988
Summary
Proline-rich protein (PRP) is a plasma protein linked to lipoproteins. This study shows PRP is an acute phase reactant, potentially crucial in metabolizing triacylglycerol-rich lipoproteins.
Area of Science:
- Biochemistry
- Immunology
- Clinical Chemistry
Background:
- Proline-rich protein (PRP) is a plasma protein associated with lipoproteins.
- Its biological significance requires further clarification.
Purpose of the Study:
- To isolate and characterize PRP.
- To investigate its biological role in plasma and its potential as an acute phase reactant.
Main Methods:
- Isolation of PRP using immunosorber column chromatography, gel filtration, and ion-exchange chromatography.
- Characterization by SDS-PAGE, isoelectric focusing, and charge shift electrophoresis.
- Quantification of PRP levels in human serum and correlation with clinical markers.
Main Results:
- PRP was identified as a pentamer with a molecular weight of 352,000.
- It contains various sugars and exhibits hydrophilic properties.
- PRP levels correlated with triacylglycerol-rich lipoproteins, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), and alpha-globulins.
- Elevated PRP levels were observed in patients with infection, inflammation, and acute myocardial infarction, paralleling ESR and CRP.
Conclusions:
- PRP functions as an acute phase reactant.
- PRP may play a significant role in the metabolism of triacylglycerol-rich lipoproteins.