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Related Experiment Videos

Induction of antigen-specific suppression by circulating Cryptococcus neoformans antigen.

J W Murphy1, R A Cox

  • 1Department of Botany and Microbiology, University of Oklahoma, Norman 73019.

Clinical and Experimental Immunology
|August 1, 1988
PubMed
Summary

Cryptococcal antigen in infected mouse serum suppresses the immune response. This study identifies cryptococcal antigen as the specific component responsible for suppressing the delayed-type hypersensitivity response to Cryptococcus neoformans.

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Area of Science:

  • Immunology
  • Microbiology
  • Infectious Diseases

Background:

  • Cryptococcus neoformans infection can lead to immunosuppression.
  • The specific component in infected mouse serum responsible for this suppression is not fully understood.

Purpose of the Study:

  • To identify the component in Cryptococcus neoformans-infected mouse serum (Inf-MS) that suppresses the delayed-type hypersensitivity (DTH) response.
  • To determine if cryptococcal antigen or anti-cryptococcal antibodies are responsible for this immunosuppression.

Main Methods:

  • Immunoaffinity chromatography using rabbit anti-cryptococcal antibody, cryptococcal antigen, and various anti-mouse immunoglobulin antibodies.
  • Quantification of cryptococcal antigen and anti-cryptococcal antibodies in serum and column fractions.

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  • Assessment of DTH responses to C. neoformans, Listeria monocytogenes, and dinitrofluorobenzene.

    • Main Results:

    • A component that suppressed the cryptococcal DTH response was adsorbed by anti-cryptococcal antibody.
    • This suppressive component correlated directly with cryptococcal antigen levels, not antibody levels.
    • The observed suppression was specific to C. neoformans and did not affect responses to other antigens.

    Conclusions:

    • Cryptococcal antigen in Inf-MS is the causative agent of antigen-specific suppression of the cell-mediated immune response to C. neoformans.
    • This finding clarifies the mechanism of immunosuppression in cryptococcal infections.