Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

2.9K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.9K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

7.5K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
7.5K
Mechanism of Angiogenesis01:10

Mechanism of Angiogenesis

6.4K
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
6.4K
MicroRNAs01:22

MicroRNAs

21.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.1K
MicroRNAs01:22

MicroRNAs

3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

6.4K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
6.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lineage-restricted dependency on an oncofetal SNHG29-IGF2BP1 RNA axis in acute megakaryoblastic leukemia.

Leukemia·2026
Same author

Density functional theory investigation of adsorption and charge transfer of fungal polyketide mycotoxins on Si, N Co-doped carbon quantum dots.

Journal of molecular modeling·2026
Same author

The long noncoding RNA <i>lnc-FAM164A1</i>-ACLY axis promotes pro-inflammatory responses in human primary macrophages: a systems approach.

Frontiers in immunology·2026
Same author

DoTools: a cross platform framework to streamline common single cell workflows.

Bioinformatics advances·2026
Same author

Risk factors for prolonged hospital stay after surgery in older patients.

Anaesthesia and intensive care·2026
Same author

Associations Between Transsphenoidal Surgery and Neuropsychiatric Disorders for Patients With Cushing's Disease.

Neurosurgery·2026

Related Experiment Video

Updated: May 2, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

27.7K

Long noncoding RNA MALAT1 regulates endothelial cell function and vessel growth.

Katharina M Michalik1, Xintian You, Yosif Manavski

  • 1From the Institute of Cardiovascular Regeneration, University Frankfurt, Frankfurt, Germany (K.M.M., Y.M., A.D., D.J., Y.P., S.U., R.A.B., S.D.); Max-Delbrück-Centrum für Molekulare Medizin (MDC), Berlin-Buch, Germany (X.Y., W.C.); Georg-Speyer-Haus, Institute for Tumor Biology and Experimental Tumor Therapy, Frankfurt, Germany (M.Z.); Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany (T.B.); and German Center for Cardiovascular Research (DZHK), Partner Site RheinMain, Frankfurt, Germany (T.B., S.U., S.D.).

Circulation Research
|March 8, 2014
PubMed
Summary

Long noncoding RNAs (lncRNAs) like metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) regulate endothelial cell function. Inhibiting MALAT1 promotes migration but reduces proliferation, impacting vascular growth in vitro and in vivo.

Keywords:
RNA, long noncodingangiogenesis effectendotheliumischemianeovascularization inhibitors

More Related Videos

Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos
07:24

Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos

Published on: December 13, 2018

5.7K

Related Experiment Videos

Last Updated: May 2, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

27.7K
Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos
07:24

Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos

Published on: December 13, 2018

5.7K

Area of Science:

  • Molecular Biology
  • Genomics
  • Cell Biology

Background:

  • The human genome contains numerous non-coding RNA sequences, including long noncoding RNAs (lncRNAs), which regulate cellular functions through various mechanisms.
  • The specific roles and expression patterns of lncRNAs within the vasculature remain largely unexplored.

Purpose of the Study:

  • To investigate the expression profile of lncRNAs in human endothelial cells.
  • To elucidate the function of the highly expressed metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in endothelial cells.

Main Methods:

  • Characterization of lncRNA expression in endothelial cells from various sources.
  • Functional studies involving silencing of MALAT1 using small interfering RNAs (siRNAs) and GapmeRs.
  • In vitro assays for cell proliferation, migration, and sprouting.
  • In vivo studies including genetic ablation of MALAT1 and pharmacological inhibition in mouse models.
  • Gene expression profiling and quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) to analyze cell cycle regulators.

Main Results:

  • Endothelial cells express high levels of conserved lncRNAs, including MALAT1, taurine upregulated gene 1 (TUG1), maternally expressed 3 (MEG3), linc00657, and linc00493.
  • Hypoxia increased MALAT1 expression, which influences endothelial cell phenotype.
  • MALAT1 silencing promoted endothelial cell migration and sprouting but inhibited proliferation in vitro.
  • In vivo, MALAT1 genetic deletion and pharmacological inhibition reduced vascularization and blood flow recovery.
  • MALAT1 silencing affected the expression of key cell cycle regulators.

Conclusions:

  • Silencing MALAT1 shifts endothelial cells from a proliferative to a migratory phenotype in vitro.
  • Genetic deletion or pharmacological inhibition of MALAT1 impairs vascular growth in vivo.