Related Experiment Video
Updated: May 2, 2026
Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma
Published on: June 2, 2023
Cytotoxic polycyclic polyprenylated acylphloroglucinols from Hypericum attenuatum
Zhong-bo Zhou1, Yang-mei Zhang2, Ke Pan2
1State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing, 210009, People's Republic of China; Xinjiang Production & Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, Tarim University, Alaer 843300, People's Republic of China.
Six new compounds, attenuatumiones A-F, were isolated from Hypericum attenuatum. Attenuatumione C demonstrated moderate cytotoxic activity against human cancer cell lines SMMC7721 and U2OS.
Area of Science:
- Natural Products Chemistry
- Medicinal Chemistry
- Pharmacognosy
Background:
- Hypericum species are known sources of bioactive compounds.
- Polycyclic polyprenylated acylphloroglucinols (PPAPs) exhibit diverse biological activities.
Purpose of the Study:
- To isolate and characterize new PPAPs from Hypericum attenuatum.
- To evaluate the cytotoxic potential of isolated compounds against human cancer cell lines.
Main Methods:
- Isolation and purification using chromatographic techniques.
- Structure elucidation via comprehensive spectroscopic analysis (NMR, MS).
- Absolute configuration determination using circular dichroism (CD) spectroscopy.
Main Results:
- Six new PPAPs, attenuatumiones A-F (1-6), were identified.
- Twelve known PPAP analogs (7-18) were also isolated.
- Attenuatumione C (3) exhibited moderate cytotoxicity against SMMC7721 and U2OS cells (IC50 values: 10.12 and 10.56 μM, respectively).
Conclusions:
- Hypericum attenuatum is a rich source of structurally diverse PPAPs.
- Attenuatumione C represents a promising lead compound for further anticancer drug development.
More Related Videos
07:16Preparation of Gynura bicolor DC samples for High-Resolution Tandem Mass Spectrometry
Published on: February 2, 2024
14:39Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults
Published on: April 22, 2022