Related Experiment Video
Updated: May 2, 2026
Mouse Abdominal Aortic Aneurysm Model Induced by Perivascular Application of Elastase
Published on: February 11, 2022
Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in
José Guilherme Minossi1, Flávio de Oliveira Lima2, Carlos Antonio Caramori3
1Sao Paulo State University, Faculty of Medicine, Departament of Surgery and Orthopedics, BotucatuSP, Brazil, PhD, Assistant Professor, Departament of Surgery and Orthopedics, Faculty of Medicine, Sao Paulo State University (UNESP), Botucatu-SP, Brazil. Conception, design, intellectual and scientific content of the study; acquisition and interpretation of data; technical procedures; manuscript writing.
Purpose:
To investigate the effects of alloxan diabetes on the abdominal wall healing of rats undergoing laparotomy.
Methods:
Ninety-six male Wistar rats weighing between 200 and 300 grams, divided into two groups: non-diabetic group (G1) and another with untreated diabetes (G2). Three months after diabetes induction, the animals underwent a 5cm-long- laparotomy and 5.0 nylon monofilament suture. After the surgery, 12 animals from each group were euthanized on days 4, 14, 21 and 30 corresponding to the moments M1, M2, M3 and M4. In each moment a fragment of the abdominal wall containing the scar was removed for tensile strength measurement, histological and morphometric study. Clinical and biochemical parameters were also analyzed.
Results:
G2 animals showed parameters compatible with severe diabetes and decreased plasma levels of insulin. The tensile strength in G2 was significantly smaller in M2 and M4, with a tendency to fall in the other two. Through light microscope, diabetic animals showed more difficulty to increase collagen density and contraction. G2 animals showed high cellularity of fibroblasts in later healing moments, with collagen thinning in M2 and M4.
Conclusion:
The abdominal wound healing in untreated diabetic animals was altered and led to a higher incidence of dehiscence and infections.
