Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Probiotics01:22

Probiotics

296
Probiotics are live, non-pathogenic microorganisms that confer health benefits by modulating the gut microbiota. The human gastrointestinal tract harbors a complex microbial ecosystem, and the balance of this microbiota is crucial for digestive and systemic health. Among the most extensively studied and utilized probiotics are species formerly classified within the genera Lactobacillus and Bifidobacterium. These organisms not only naturally colonize the human gut but are also consumed through...
296
Development of Human Microbiota01:30

Development of Human Microbiota

61
The human microbiota begins developing at birth and undergoes continual change as we age. Infancy marks a critical period of microbial sensitivity, offering a “window of opportunity” during which beneficial microbes help mature the immune system. By age three, children typically develop a more stable and diverse microbial community. Newborns acquire microbes from their immediate environment; vaginal delivery favors maternal vaginal microbes, while cesarean births favor microbes from...
61
Production of Antibiotics01:27

Production of Antibiotics

265
Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...
265
Microbes in the Production of Fermented Foods01:27

Microbes in the Production of Fermented Foods

327
Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
327
Production of Organic Acids01:25

Production of Organic Acids

105
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
105
Bioavailability Enhancement: Drug Stability Enhancement and GI Retention01:05

Bioavailability Enhancement: Drug Stability Enhancement and GI Retention

332
Improving a drug's stability in the gastrointestinal (GI) tract is paramount for enhancing its bioavailability and therapeutic effectiveness. Various strategies are employed to protect the drug from the harsh gastric milieu and to ensure its release and absorption at the desired site within the GI tract.Polymer coatings are one such method used to shield drugs from the stomach's acidic environment. By preventing premature drug release, these coatings improve the bioavailability of unstable...
332

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessment of the Safety of Anti-Salmonella Disinfectant for Veterinary Use Based on a Cocktail of Bacteriophages.

Bulletin of experimental biology and medicine·2024
Same author

Isolation and Characterization of Salmonella Bacteriophages as Potential Agents for Phage Therapy of Antibiotic-Resistant Intestinal Infections.

Bulletin of experimental biology and medicine·2024
Same author

[Microbiota markers level in the blood and cerebrospinal fluid of patients with different types of multiple sclerosis and radiologically isolated syndrome].

Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova·2023
Same author

[Development and preservation of specific T-cell immunity after COVID-19 or vaccination against this infection].

Voprosy virusologii·2023
Same author

Composition of Bacteriophages for Therapeutic and Prophylactic Use in Monkeys.

Bulletin of experimental biology and medicine·2023
Same author

Features of the Humoral Response to Infection, Vaccination, and Revaccination during COVID-19.

Bulletin of experimental biology and medicine·2022

Related Experiment Video

Updated: May 2, 2026

Author Spotlight: Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality
05:45

Author Spotlight: Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality

Published on: April 7, 2023

4.5K

[Development of polycomponent metabolite probiotic].

Iu V Kulakova, A V Aleshkin, S S Afanas'ev

    Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
    |March 11, 2014
    PubMed
    Summary

    A novel polycomponent probiotic agent was developed using Lactobacillus supernatant, retaining antimicrobial and antifungal activity without live microorganisms. This metabolite probiotic offers a stable alternative to traditional probiotic compositions.

    Area of Science:

    • Microbiology
    • Biotechnology
    • Probiotics

    Background:

    • Development of stable and effective probiotic formulations is crucial for therapeutic applications.
    • Lactobacilli are key components in many probiotic products, known for their health benefits.
    • Utilizing cultural fluid supernatant offers a potential avenue for cell-free probiotic agents.

    Purpose of the Study:

    • To construct a polycomponent probiotic composition utilizing lactobacilli cultural fluid (CF) supernatant.
    • To evaluate the probiotic effect and metabolite profiles of CF supernatant compared to native cultures.
    • To develop a technology for producing an antimicrobial probiotic agent based on CF.

    Main Methods:

    • Lactobacilli strain identification using 16S RNA gene sequencing.

    More Related Videos

    Novel Production Protocol for Small-scale Manufacture of Probiotic Fermented Foods
    08:38

    Novel Production Protocol for Small-scale Manufacture of Probiotic Fermented Foods

    Published on: September 10, 2016

    25.4K
    Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation
    09:49

    Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation

    Published on: October 31, 2019

    23.7K

    Related Experiment Videos

    Last Updated: May 2, 2026

    Author Spotlight: Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality
    05:45

    Author Spotlight: Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality

    Published on: April 7, 2023

    4.5K
    Novel Production Protocol for Small-scale Manufacture of Probiotic Fermented Foods
    08:38

    Novel Production Protocol for Small-scale Manufacture of Probiotic Fermented Foods

    Published on: September 10, 2016

    25.4K
    Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation
    09:49

    Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation

    Published on: October 31, 2019

    23.7K
  • Comparative analysis of CF supernatant, ultrafiltrate, and native cultures for peptide, amino acid, and organic acid content.
  • Assessment of bactericidal and fungicidal activity against target microorganisms (Staphylococcus aureus, Enterococcus faecalis, Candida albicans).

    • Main Results:

    • A technology for producing an amicrobial probiotic agent based on lactobacilli CF supernatant was successfully developed.
    • CF supernatant demonstrated analogous protein metabolite concentrations and an identical set of organic acids compared to native lactobacilli cultures.
    • Both CF supernatant and native lactobacilli cultures exhibited equivalent bactericidal and anticandidosis activity against tested strains.

    Conclusions:

    • The polycomponent metabolite probiotic, based on CF supernatant of specific Lactobacillus strains, effectively retains the antimicrobial and fungicidal activity of the native consortium.
    • This supernatant-based probiotic offers advantages over compositions containing live microorganisms, potentially enhancing stability and shelf-life.
    • The developed agent represents a promising cell-free probiotic alternative with retained biological efficacy.