Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

3.4K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Novel approaches for neuroprotection: Focus on neurodegenerative and ischemic central nervous system diseases.

Neuroprotection (Chichester, England)·2026
Same author

Clinical Predictors of Survival After Palliative Radiotherapy for Glioblastoma in a Real-World Cohort Study.

Current oncology (Toronto, Ont.)·2026
Same author

Real-World Survival Outcomes Following Chemoradiotherapy with or Without Durvalumab in PD-L1-Defined Subgroups of Stage III Unresectable NSCLC.

Current oncology (Toronto, Ont.)·2026
Same author

Neurofilament light and glial fibrillary acidic protein do not reflect neuronal or glial damage during different intracranial radiotherapy regimes: a pilot study.

Frontiers in oncology·2026
Same author

Deformable image registration accuracy: impact of user-defined parameter selection on contour propagation for deep inspiration breath-hold and free breathing breast radiotherapy.

BMC medical imaging·2026
Same author

Editorial: Recent advances in translational neurovascular and cerebroprotection research.

Frontiers in neuroscience·2026

Related Experiment Video

Updated: May 2, 2026

Bone Marrow Transplantation Procedures in Mice to Study Clonal Hematopoiesis
08:00

Bone Marrow Transplantation Procedures in Mice to Study Clonal Hematopoiesis

Published on: May 26, 2021

12.9K

Modelling hematological parameters after total body irradiation.

Christopher Oelkrug1, Nadja Hilger, Uta Schönfelder

  • 1Fraunhofer Institute for Cell Therapy and Immunology (IZI) , Leipzig , Germany.

International Journal of Radiation Biology
|March 11, 2014
PubMed
Summary

Radiation dose impacts the timing but not the speed of blood cell recovery. Higher doses delay hematopoietic recovery, but all cell types eventually reach similar levels, aiding stem cell research.

Keywords:
Hematopoiesisradiationtotal body irradiation (TBI)transplantation

More Related Videos

In Vivo Osteo-organoid Approach for Harvesting Therapeutic Hematopoietic Stem/Progenitor Cells
05:32

In Vivo Osteo-organoid Approach for Harvesting Therapeutic Hematopoietic Stem/Progenitor Cells

Published on: February 16, 2024

1.2K
Intestinal Epithelial Regeneration in Response to Ionizing Irradiation
09:10

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

Published on: July 27, 2022

2.4K

Related Experiment Videos

Last Updated: May 2, 2026

Bone Marrow Transplantation Procedures in Mice to Study Clonal Hematopoiesis
08:00

Bone Marrow Transplantation Procedures in Mice to Study Clonal Hematopoiesis

Published on: May 26, 2021

12.9K
In Vivo Osteo-organoid Approach for Harvesting Therapeutic Hematopoietic Stem/Progenitor Cells
05:32

In Vivo Osteo-organoid Approach for Harvesting Therapeutic Hematopoietic Stem/Progenitor Cells

Published on: February 16, 2024

1.2K
Intestinal Epithelial Regeneration in Response to Ionizing Irradiation
09:10

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

Published on: July 27, 2022

2.4K

Area of Science:

  • Hematology
  • Radiation Biology
  • Stem Cell Biology

Background:

  • Hematopoiesis, the process of blood cell formation, is sensitive to radiation exposure.
  • Understanding stem cell population dynamics is crucial for predicting hematopoietic recovery.
  • This knowledge can inform the development of advanced stem cell transplantation models.

Purpose of the Study:

  • To investigate the time- and dose-dependent reconstitution of hematopoiesis post-irradiation.
  • To establish predictive models for hematological parameters based on radiation exposure.
  • To gain insights into the hematopoietic system for potential therapeutic applications.

Main Methods:

  • CD4-/- C57Bl/6 mice (transgenic for human CD4 and HLA-DR3) were subjected to single (3-12 Gy) and fractionated irradiation regimens.
  • Weekly blood analyses monitored red blood cells (RBC), hemoglobin (Hb), hematocrit (HCT), and white blood cells (WBC).
  • Histopathology examined organ and tissue damage, including bone marrow cellularity.

Main Results:

  • Hematopoietic recovery (RBC, Hb, HCT, WBC) occurred within one week across all doses but initiated at dose-dependent times.
  • Higher radiation doses resulted in a delayed start to hematopoietic recovery.
  • Red blood cell, hemoglobin, and hematocrit levels recovered to dose-independent saturation concentrations.
  • Single-dose irradiation caused dose-dependent bone marrow hypocellularity; fractionated doses led to bone marrow regeneration.

Conclusions:

  • Mathematical functions were developed to model hematopoietic parameter reconstitution after total body irradiation.
  • The timing of recovery is dose-dependent, while recovery velocity and final levels are largely independent of dose.
  • Findings support the use of hematological parameters for predicting radiation effects and advancing stem cell research.