Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Designed gene amplification on the Bacillus subtilis chromosome.

M Mori1, K Hashiguchi, K Yoda

  • 1Department of Agricultural Chemistry, University of Tokyo, Japan.

Journal of General Microbiology
|January 1, 1988
PubMed
Summary

Researchers developed a novel method for gene amplification in Bacillus subtilis, successfully increasing the copy number of a 22 kb DNA region. This technique enhances the production of key enzymes like alpha-amylase and shikimate kinase.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reproductive characteristics in an understory bamboo and gradual environmental changes after its dieback provide an extended opportunity for overstory tree regeneration in a mixed cool-temperate forest in central Japan.

Plant biology (Stuttgart, Germany)·2023
Same author

The impact of omalizumab on paid and unpaid work productivity among severe Japanese cedar pollinosis (JCP) patients.

Journal of medical economics·2022
Same author

Emotion regulation processes and middle-aged Japanese women's health.

Climacteric : the journal of the International Menopause Society·2020
Same author

A randomized controlled phase II clinical trial comparing ONO-4053, a novel DP1 antagonist, with a leukotriene receptor antagonist pranlukast in patients with seasonal allergic rhinitis.

Allergy·2017
Same author

Distinct Interaction of TmrB Protein with Membranes in Bacillus subtilis and Escherichia coli.

Bioscience, biotechnology, and biochemistry·2016
Same author

Induction of Two Types of Gene Amplification by a Cloned DNA Fragment and Amplification of a Foreign Gene on the Bacillus subtilis Chromosome by Using the Fragment.

Bioscience, biotechnology, and biochemistry·2016

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biotechnology

Background:

  • Bacillus subtilis strain B7 exhibits tandem amplification of chromosomal DNA regions.
  • Previous work involved cloning a 1.6 kb fragment to induce gene amplification.

Purpose of the Study:

  • To design and implement a gene amplification system for a 22 kb region on the Bacillus subtilis chromosome.
  • To enhance the production of alpha-amylase and shikimate kinase through targeted gene amplification.

Main Methods:

  • Cloning of DNA fragments from the termini of the 22 kb region.
  • Construction of a designed repeating unit on the pBR327 plasmid.
  • Transformation of Bacillus subtilis wild-type strain with the constructed plasmid.

Related Experiment Videos

Main Results:

  • Successful induction of 22 kb gene amplification in tunicamycin-resistant transformants.
  • Demonstrated high productivity of alpha-amylase and shikimate kinase in engineered strains.
  • Estimated 10-20 copies of the amplified repeating unit per chromosome.

Conclusions:

  • A novel system for targeted gene amplification of large DNA regions (>20 kb) in Bacillus subtilis has been established.
  • This method offers potential for improving the yield of industrially relevant enzymes.