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Updated: May 2, 2026
Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages
Published on: August 12, 2019
Peripheral monocytes of obese women display increased chemokine receptor expression and migration capacity
P Krinninger1, R Ensenauer, K Ehlers
1Else Kroener-Fresenius Center for Nutritional Medicine (P.K., K.E., K.R., J.S., H.H., H.L.), Department of Nutritional Medicine, ZIEL Research Centre for Nutrition and Food Sciences, Clinical Cooperation Group Nutrigenomics and Type 2 Diabetes (K.E., H.H., H.L.), Helmholtz Zentrum München and Technical University München, and Else Kroener-Fresenius-Center for Nutritional Medicine (H.H.), Klinikum Rechts der Isar, Technische Universität München, 85350 Freising-Weihenstephan, Germany; German Center for Diabetes Research, 85764 Neuherberg, Germany; Research Center (R.E.), Dr von Hauner Children's Hospital, Ludwig-Maximilians-Universität München, 80337 München, Germany; Comprehensive Pneumology Center (S.K.-E.), Helmholtz Zentrum München, Ludwig-Maximilians University, and Asklepios Clinic Gauting, 82131 München, Germany.
Context:
The activation of peripheral immune cells and the infiltration of immune cells into adipose tissue in obesity are implicated in the development of type 2 diabetes mellitus.
Objective:
The aim of the study was to compare peripheral immune cells from obese and normal-weight women with regard to composition of immune cell subpopulations, surface expression of the chemokine receptors (CCRs) CCR2, CCR3, CCR5, and CXCR3 (chemokine (C-X-C motif) receptor 3) and cell-intrinsic migration capacity.
Design:
This was a case-control study.
Setting:
The study was conducted at a university clinical study center.
Patients:
Obese females and normal-weight females were included for fluorescence-activated cell sorting analysis and migration assays.
Main Outcome Measures:
Peripheral blood mononuclear cells were prepared from fasting blood samples and used for fluorescence-activated cell sorting analysis and migration assays.
Results:
An increase in the percentages of CD14(+)CD16(+) monocytes was observed in obese subjects compared with controls. The CCR profile of monocytes differed significantly in the obese state; in particular, CCR2 levels were increased. In addition, a higher chemotactic activity of monocytes from obese subjects was observed in a migration assay, which was associated with both insulin resistance and CCR2 expression.
Conclusion:
Our results suggest that the enhanced intrinsic migratory capacity of peripheral monocytes in obese women may be due to the increased CCR expression, further supporting a link between peripheral immune cell dysfunction and obesity.
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