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Design of clone-specific probes from genome sequences for rapid PCR-typing of outbreak pathogens
E López-Camacho1, Z Rentero, G Ruiz-Carrascoso
1Servicio de Microbiología, Hospital Universitario La Paz, IdiPAZ, Madrid, Spain.
Abstract:
The genome sequence of one OXA-48-producing Klebsiella pneumoniae belonging to sequence type (ST) 405, and three belonging to ST11, were used to design and test ST-specific PCR assays for typing OXA-48-producing K. pneumoniae. The approach proved to be useful for in-house development of rapid PCR typing assays for local outbreak surveillance.

