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Urease-based ISFET biosensor for arginine determination.

M Sheliakina1, V Arkhypova2, O Soldatkin3

  • 1Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Zabolotnogo Street 150, 03143 Kyiv, Ukraine; Max Planck Institute for Polymer Research, Ackermannweg 10, D-55128 Mainz, Germany.

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Summary

A novel biosensor utilizing urease inhibition effectively determines arginine levels. This ion-selective field-effect transistor (ISFET) based device offers sensitive and reproducible arginine quantification in real samples.

Keywords:
ArginineBiosensorInhibitionIon-selective field effect transistorsUrease

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Area of Science:

  • Biotechnology
  • Biosensor Technology
  • Analytical Chemistry

Background:

  • Arginine is a crucial amino acid with implications in various physiological processes.
  • Accurate determination of arginine is essential for clinical diagnostics and nutritional analysis.
  • Existing methods for arginine detection may have limitations in sensitivity or sample applicability.

Purpose of the Study:

  • To develop and validate a novel biosensor for the quantitative determination of arginine.
  • To investigate the urease inhibition effect as a basis for arginine detection.
  • To assess the performance characteristics of the proposed biosensor, including sensitivity, selectivity, and reproducibility.

Main Methods:

  • Development of a biosensor using ion-selective field-effect transistors (ISFETs) as transducers.
  • Immobilization of urease enzyme in glutaraldehyde vapor as the biorecognition element.
  • Optimization of urea concentration for maximal response to arginine inhibition.
  • Evaluation of biosensor selectivity against various amino acids.

Main Results:

  • The biosensor demonstrated effective arginine determination based on the urease inhibition principle.
  • An optimal urea concentration was identified for sensitive arginine detection.
  • The limit of detection for arginine was determined to be 0.05 mM.
  • The biosensor exhibited good selectivity towards different amino acids.
  • Quantitative analysis of l-arginine in a real-world sample ("Arginine Veyron") showed high accuracy (5.2% relative error).
  • The biosensor provided reproducible arginine determination results.

Conclusions:

  • The developed ISFET-based biosensor offers a sensitive and selective method for arginine determination.
  • The urease inhibition approach is a viable strategy for constructing arginine biosensors.
  • The biosensor shows potential for application in analyzing arginine content in various samples, including nutritional supplements.