Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Non-disruptive in vitro monitoring of cellular states with cell-free DNA methylation.

Genome biology·2026
Same author

Unmasking metabolic disruptors: The NEMESIS project's quest for Novel Biomarkers, Evidence on Adverse Effects, and Efficient Methodologies.

Open research Europe·2026
Same author

Author Correction: Towards a reference cell atlas of liver diversity over the human lifespan.

Nature reviews. Gastroenterology & hepatology·2025
Same author

Towards a reference cell atlas of liver diversity over the human lifespan.

Nature reviews. Gastroenterology & hepatology·2025
Same author

Wnt signalling maintains self-renewal of human hepatoblasts without blocking their differentiation.

Development (Cambridge, England)·2025
Same author

Transcriptomic and epigenetic mechanisms controlling cholangiocyte transdifferentiation into hepatocytes.

Journal of hepatology·2025

Related Experiment Video

Updated: Feb 28, 2026

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells
07:37

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

Published on: June 11, 2019

9.2K

Heps with pep: direct reprogramming into human hepatocytes.

Ludovic Vallier1

  • 1Wellcome Trust-Medical Research Council Stem Cell Institute, Anne McLaren Institute for Regenerative Medicine, Department of Surgery, West Forvie Site, Robinson Way, Cambridge CB20SZ, UK; Wellcome Trust Sanger Institute, Hinxton CB10 1SA, UK.

Cell Stem Cell
|March 11, 2014
PubMed
Summary

Scientists have developed a new method to create liver cells from skin cells. These induced hepatocytes show promise for drug development and treating liver diseases.

More Related Videos

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery
12:40

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery

Published on: May 19, 2018

10.8K
Robust Generation of Hepatocyte-like Cells from Human Embryonic Stem Cell Populations
05:49

Robust Generation of Hepatocyte-like Cells from Human Embryonic Stem Cell Populations

Published on: October 26, 2011

48.5K

Related Experiment Videos

Last Updated: Feb 28, 2026

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells
07:37

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

Published on: June 11, 2019

9.2K
Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery
12:40

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery

Published on: May 19, 2018

10.8K
Robust Generation of Hepatocyte-like Cells from Human Embryonic Stem Cell Populations
05:49

Robust Generation of Hepatocyte-like Cells from Human Embryonic Stem Cell Populations

Published on: October 26, 2011

48.5K

Area of Science:

  • Hepatology
  • Stem Cell Biology
  • Regenerative Medicine

Background:

  • Primary human hepatocytes are crucial for drug metabolism studies and liver disease therapies.
  • Their limited availability and expansion capacity pose significant challenges in pharmaceutical research and clinical applications.

Purpose of the Study:

  • To demonstrate efficient direct reprogramming of human fibroblasts into induced hepatocytes.
  • To assess the functional and metabolic characteristics of these reprogrammed cells.

Main Methods:

  • Direct reprogramming of human fibroblasts into induced hepatocytes.
  • Assessment of metabolic functions and gene expression profiles of induced hepatocytes.

Main Results:

  • Successful direct reprogramming of human fibroblasts into induced hepatocytes.
  • Induced hepatocytes exhibit metabolic properties comparable to primary human hepatocytes.

Conclusions:

  • Direct reprogramming offers a viable alternative to overcome the limitations of primary human hepatocytes.
  • Induced hepatocytes hold potential for pharmaceutical applications and cell-based liver therapies.