Related Experiment Video
Updated: May 2, 2026
Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures
Published on: January 9, 2019
Assessment of expression of epidermal growth factor receptor and p53 in meningiomas
Swetha Narla, Megha S Uppin, M Vijaya Saradhi
1Department of Pathology, Nizam's Institute of Medical Sciences, Hyderabad, Andhra Pradesh, India.
Background:
Meningiomas represent about 30% of primary adult central nervous system tumors. Though slow growing, they recur, causing significant morbidity and mortality.
Objective:
The objective of the following study is to grade meningiomas according to World Health Organization (WHO) 2007 criteria and to correlate the grade with degree of expression of epidermal growth factor receptor (EGFR) and p53.
Materials And Methods:
Meningiomas diagnosed in the year 2010 in the Department of Pathology of our institute, were included in the study. Clinical and radiological findings were noted from medical records. The histopathology slides were reviewed and the tumors were graded according to WHO 2007 criteria. Tissue microarrays (TMA) were prepared and immunohistochemical analysis with epithelial membrane antigen, Vimentin, Ki67, EGFR and p53 was performed on the TMA slides.
Results:
A total of 79 meningiomas diagnosed during the study period included 30 male and 49 female patients with an age range of 10-75 years. There was a female preponderance with M:F ratio of 1:1.63. EGFR was found to be higher in grade I (82.93%) compared with grade II (35.71%) and grade III tumors (20%) with an overall positivity of 60.81%. Mean p53 indices were higher in grade III (50%) compared with grade II (39.29%) and grade I tumors (38.46%) with an overall positivity of 39.44%. Ki67 labeling index (LI) was significantly high in grade III (16.4%) compared with grade II (6.46%) and grade I tumors (3.13%).
Conclusion:
EGFR expression and Ki67 LI correlated with grade of meningioma P < 0.0001 and P < 0.0001 respectively which were statistically significant whereas p53 expression did not correlate (P - 0.90).
Insights
Epidermal growth factor receptor (EGFR) expression and Ki67 labeling index correlate with meningioma grade, but p53 expression does not. This study graded meningiomas using WHO 2007 criteria and analyzed protein expression.
Area of Science:
- Neuro-oncology
- Tumor biology
- Pathology
Background:
- Meningiomas are common primary central nervous system tumors, accounting for approximately 30% of adult cases.
- Despite slow growth, meningiomas can recur, leading to significant patient morbidity and mortality.
Purpose of the Study:
- To grade meningiomas based on World Health Organization (WHO) 2007 criteria.
- To investigate the correlation between tumor grade and the expression levels of epidermal growth factor receptor (EGFR) and p53.
Main Methods:
- Histopathological review and grading of 79 meningiomas diagnosed in 2010 according to WHO 2007 criteria.
- Immunohistochemical analysis of tissue microarrays (TMA) for EGFR, p53, and Ki67 expression.
- Correlation of protein expression with tumor grade and clinical/radiological findings.
Main Results:
- Epidermal growth factor receptor (EGFR) expression was highest in grade I meningiomas (82.93%) and decreased in higher grades.
- Mean p53 indices and Ki67 labeling index (LI) were significantly higher in grade III meningiomas compared to grades I and II.
- Ki67 LI showed a significant increase with increasing meningioma grade.
Conclusions:
- EGFR expression and Ki67 LI are statistically significant indicators correlating with meningioma grade (P < 0.0001).
- p53 expression did not show a significant correlation with meningioma grade (P = 0.90).
- These findings highlight the potential of EGFR and Ki67 as biomarkers for meningioma grading.
More Related Videos
Related Concept Videos
02:23Abnormal Proliferation
02:38Mitogens and the Cell Cycle