Phytosterols elevation in bamboo shoot residue through laboratorial scale solid-state fermentation using isolated...

Area of Science:

• Biotechnology
• Microbial Fermentation
• Natural Product Chemistry

Background:

• Bamboo shoot residue is a lignocellulosic waste material.
• Phytosterols are valuable plant compounds with health benefits.
• Current methods for phytosterol extraction can be inefficient or costly.

Purpose of the Study:

• To enhance phytosterol content in bamboo shoot residue using solid-state fermentation (SSF).
• To optimize fermentation conditions for phytosterol production by Aspergillus niger CTBU.
• To evaluate the potential for scaling up the process for industrial application.

Main Methods:

• Isolation and acclimatization of Aspergillus niger CTBU from local bamboo shoot residue.
• Optimization of fermentation temperature and nitrogen levels for SSF.
• Conducting SSF in Erlenmeyer flasks with optimized parameters and analyzing phytosterol content via HPLC.

Main Results:

• The optimized SSF process achieved 1,186 mg/100g of total phytosterols, a significant increase from 523 mg/100g in fresh residue.
• HPLC analysis confirmed the stability of phytosterol types and composition ratios.
• Acclimatization of the strain improved fermentation performance.

Conclusions:

• Solid-state fermentation with Aspergillus niger CTBU is an effective method to elevate phytosterol content in bamboo shoot residue.
• The optimized SSF process demonstrates potential for cost-effective, large-scale production of phytosterols from agricultural waste.
• This approach contributes to valorizing discarded bamboo shoot residue.