Miniaturization of mitotic index cell-based assay using "wall-less" plate technology
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Updated: May 2, 2026
A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
Published on: June 16, 2016
Xavier Le Guezennec1, Mark Phong, Liyana Nor
11 Curiox Biosystems Pte Ltd. , Singapore .
Abstract:
The use of microscopic imaging for the accurate assessment of cells in mitosis is hampered by the round morphology of mitotic cells, which renders them poorly adherent and highly susceptible to loss during the washing stage of cell-based assays. Here, to circumvent these limitations, we make use of DropArray, a recent technology that allows high retention of weakly adherent cells and suspension cells. DropArray offers the competitive advantage of maintaining the classic high throughput format of microtiter plates while reducing classic microwell volume by up to 90% by using a drop format. Here, we present a mitotic index cell-based assay using the mitosis marker phospho histone H3 at serine 10 on a DropArray 384-well plate format. Dose-response curve analysis of the mitotic index assay with an antimitotic drug (docetaxel) on DropArray is presented that shows an effective dosage compared to previous established results similar to those obtained with conventional microtiter plates. The mitotic index assay with DropArray showed a Z-factor >0.6. Our results validate DropArray as a suitable platform for high throughput screening for compounds affecting mitosis or the cell cycle.
Insights
This study introduces DropArray, a novel technology for improved cell retention in mitosis assays. DropArray enhances high-throughput screening for compounds impacting cell division and the cell cycle.
Area of Science:
- Cell Biology
- Assay Development
- High-Throughput Screening
Background:
- Assessing cells in mitosis via microscopic imaging is challenging due to poor cell adhesion and loss during washing stages.
- Conventional microtiter plates struggle with retaining weakly adherent and suspension cells, impacting assay accuracy.
Purpose of the Study:
- To evaluate DropArray, a new technology, for its efficacy in improving cell retention for mitotic index assays.
- To validate DropArray as a platform for high-throughput screening of compounds affecting mitosis and cell cycle progression.
Main Methods:
- Development of a mitotic index cell-based assay using phospho histone H3 (serine 10) as a marker.
- Utilizing the DropArray 384-well plate format for enhanced retention of weakly adherent and suspension cells.
- Performing dose-response curve analysis with docetaxel to assess assay effectiveness.
Main Results:
- DropArray demonstrated high retention of cells, overcoming limitations of conventional assays.
- The mitotic index assay on DropArray showed a Z-factor >0.6, indicating assay robustness.
- Dose-response analysis with docetaxel yielded effective dosage results comparable to conventional methods.
Conclusions:
- DropArray is a suitable platform for high-throughput screening of compounds affecting mitosis.
- The technology enhances cell retention, improving the accuracy and reliability of cell-based assays.
- DropArray offers a competitive advantage by maintaining high-throughput capabilities with reduced well volumes.
