Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Vesicular Tubular Clusters01:45

Vesicular Tubular Clusters

2.4K
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
2.4K
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

8.1K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
8.1K
Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

3.1K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
3.1K
COP Coated Vesicles00:59

COP Coated Vesicles

12.6K
Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of...
12.6K
Intralumenal Vesicles and Multivesicular Bodies01:38

Intralumenal Vesicles and Multivesicular Bodies

4.0K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
4.0K
Fusion of Secretory Vesicles with the Plasma Membrane01:26

Fusion of Secretory Vesicles with the Plasma Membrane

15.9K
Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
15.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of physiological strain on lung epithelial cells by exposure to aerosolised quartz silica in a perfused bioreactor.

Frontiers in bioengineering and biotechnology·2026
Same author

Advancing preclinical research with reconstructed in vitro skin models mimicking non-healing wounds.

International journal of pharmaceutics: X·2026
Same author

Exploring the Potential Role of Manganese-Based Zeolitic Imidazolate Framework Nanoparticles in Cancer Therapy: <i>In vitro</i> Studies Using Lung Cancer Cells.

International journal of nanomedicine·2026
Same author

Alignment of in vitro and in vivo pulmonary inflammation models using crystalline quartz silica.

Particle and fibre toxicology·2026
Same author

Long-term exposure to nanoparticles alters senescence-associated markers and immune responses in human monocyte-derived macrophages.

Nanoscale·2026
Same author

Safety and anti-inflammatory activity of Gentiana lutea L. in human bronchial epithelial cell cultures.

Journal of ethnopharmacology·2026

Related Experiment Video

Updated: May 2, 2026

Co-Translational Insertion of Membrane Proteins into Preformed Nanodiscs
08:24

Co-Translational Insertion of Membrane Proteins into Preformed Nanodiscs

Published on: November 19, 2020

3.0K

Insertion of nanoparticle clusters into vesicle bilayers.

Cécile Bonnaud1, Christophe A Monnier, Davide Demurtas

  • 1Adolphe Merkle Institute, University of Fribourg , Route de l'Ancienne Papéterie CP209, 1723 Marly 1, Switzerland.

ACS Nano
|March 12, 2014
PubMed
Summary

Researchers overcame nanoparticle size limitations in liposome-nanoparticle hybrids by clustering nanoparticles before vesicle formation. This technique allows for the incorporation of larger structures, up to 60 nm, enhancing nanobiotechnology applications.

More Related Videos

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
10:34

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Published on: April 23, 2017

6.4K
Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins
11:30

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins

Published on: August 31, 2019

27.0K

Related Experiment Videos

Last Updated: May 2, 2026

Co-Translational Insertion of Membrane Proteins into Preformed Nanodiscs
08:24

Co-Translational Insertion of Membrane Proteins into Preformed Nanodiscs

Published on: November 19, 2020

3.0K
Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
10:34

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Published on: April 23, 2017

6.4K
Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins
11:30

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins

Published on: August 31, 2019

27.0K

Area of Science:

  • Materials Science
  • Nanotechnology
  • Biotechnology

Background:

  • Current liposome-nanoparticle hybrid development is limited by the small size (approx. 6.5 nm) of nanoparticles that can be incorporated within vesicle bilayers.
  • This size constraint restricts the versatility and application scope of these hybrid materials.

Purpose of the Study:

  • To investigate a method for increasing the size of nanoparticles that can be incorporated into liposome membranes.
  • To overcome the existing size limitations in liposome-nanoparticle hybrid formation.

Main Methods:

  • Experimental observations combined with theoretical considerations.
  • Cryo-transmission electron microscopy (Cryo-TEM) and cryo-electron tomography (Cryo-ET) for detailed structural analysis.

Main Results:

  • Promoting nanoparticle clustering before liposome formation enables the incorporation of larger structures within vesicle membranes.
  • Cryo-TEM and Cryo-ET confirmed that liposome membranes can accommodate flexible structures up to 60 nm in size.
  • Demonstrated a significant increase in the inclusion capability of liposome membranes.

Conclusions:

  • The developed method significantly enhances the versatility of liposome-nanoparticle hybrids.
  • This advancement opens new avenues for developing multivalent vesicles for diverse nanobiotechnology applications.