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Characterization of receptor for granulocyte colony-stimulating factor on human circulating neutrophils
1Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Biochemical and Biophysical Research Communications
|October 31, 1988
Abstract:
We have made a mutein of human G-CSF with more stable, and potent biological activity. Using 125 I-labeled mutein human G-CSF, high affinity binding sites were identified on human circulating neutrophils. Receptor number per cell was 560 with a Kd of 250 pM. The human G-CSF receptor was identified as a single subunit protein of Mr approximately 150,000.