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Updated: May 2, 2026

Isolation of Double Negative αβ T Cells from the Kidney
Published on: May 16, 2014
Ex vivo converted double negative T cells suppress activated B cells
WenXia Li1, Yue Tian2, Zhao Li1
1Department of Hepatobiliary Surgery, Peking University People's Hospital, NO. 11, Xizhimen South Street, Xicheng District, Beijing 100044, China.
Abstract:
Although the ability of endogenous CD4(-)CD8(-) double negative (DN) T cells to suppress B cells has been documented, the extent to which ex vivo converted DN T cells suppress B cells activity is still being explored. The aim of this study was to determine whether and what extent ex vivo converted CD4(-)CD8(-) DN T cells suppress B cell activation and antibody production. We found that ex vivo converted DN T cells suppressed proliferation of activated B cells in a perforin and cell-cell contact dependent manner. In addition, ex vivo converted DN T cells significantly inhibited the production of IgG by stimulated B cells. This study provides evidence that ex vivo converted CD4(-)CD8(-) double negative T cells can down-regulate immune responses by suppressing B cell proliferation and IgG production, and supports efforts to develop ex vivo DN T cell therapies.
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