Related Experiment Video

Updated: May 2, 2026

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

9.3K

Differential effects of nitrostyrene derivatives on myelopoiesis involve regulation of C/EBPα and p38MAPK activity

Marije Bartels1, Andrana K Calgarotto2, Anton C Martens3

  • 1Department of Cell Biology, University Medical Center Utrecht, Utrecht, The Netherlands; Division of Pediatrics, University Medical Center Utrecht, Utrecht, The Netherlands.

Plos One
|March 12, 2014
PubMed

Insights

Nitrostyrene derivatives show promise in treating bone marrow failure syndromes by enhancing myeloid progenitor cell expansion and differentiation. These compounds improve myeloid reconstitution after chemotherapy, offering new therapeutic avenues.

Area of Science:

  • Hematology
  • Oncology
  • Pharmacology

Background:

  • Bone marrow failure syndromes and myelodysplastic syndromes (MDS) are heterogeneous diseases with ineffective myelopoiesis and poor treatment responses.
  • Nitrostyrene derivatives are known protein phosphatase inhibitors with pro-apoptotic effects in tumor cells, but their impact on normal hematopoietic cells is largely unknown.

Purpose of the Study:

  • To investigate the effects of nitrostyrene derivatives (NTS1 and NTS2) on myeloid development.
  • To evaluate their potential as therapeutic agents for bone marrow failure and related conditions.

Main Methods:

  • Utilized umbilical cord blood-derived myeloid progenitor cells and patient-derived bone marrow cells.
  • Employed a (BALB/c) mouse model for in vivo studies.
  • Assessed myeloid progenitor expansion, differentiation, and hematopoietic reconstitution post-chemotherapy.

Main Results:

  • Nitrostyrene derivatives stimulated myeloid progenitor expansion and differentiation in vitro.
  • Compounds improved myeloid reconstitution in vivo after chemotherapy-induced bone marrow depletion.
  • Observed increased C/EBPα expression/activity and p38MAPK pathway inhibition.

Conclusions:

  • Nitrostyrenes enhance myelopoiesis and represent potential treatments for bone marrow failure syndromes, hypocellular MDS, and chemotherapy-induced aplasia.
  • Further research into nitrostyrenes could lead to novel therapeutic strategies for hematologic disorders.

More Related Videos

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation11:40

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

Published on: October 20, 2014

8.1K
Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants07:38

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

951

Related Concept Videos

Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.4K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
4.7K
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.5K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.0K
Role of Hematopoietic Growth Factors01:28

Role of Hematopoietic Growth Factors

Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...
3.9K
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
3.1K