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Retinoids modulate thioacetamide-induced acute hepatotoxicity.

Igor O Shmarakov¹, Vira L Borschovetska, Mykhailo M Marchenko

¹Department of Biochemistry and Biotechnology, Chernivtsi National University, Chernivtsi 58000, Ukraine.

Toxicological Sciences : an Official Journal of the Society of Toxicology

|March 12, 2014

Summary

Lifetime hepatic retinoid stores significantly influence xenobiotic metabolism and toxicity. Mice lacking these stores (Lrat(-/-)) showed no thioacetamide (TAA) induced liver injury, unlike wild-type mice, demonstrating retinoids

Keywords:

gavage hepatic stellate cell retinoic acid retinyl ester vitamin A

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Area of Science:

Hepatology
Toxicology
Biochemistry

Background:

Retinoids and xenobiotics mutually influence each other's metabolism and actions.
The impact of lifelong hepatic retinoid accumulation on xenobiotic metabolism remains poorly understood.

Purpose of the Study:

To investigate how endogenous hepatic retinoid stores affect xenobiotic metabolism and toxicity.
To determine the role of retinoid availability in thioacetamide (TAA)-induced liver injury.

Main Methods:

Hepatotoxicity was induced using thioacetamide (TAA) in wild-type (WT) mice and lecithin:retinol acyltransferase-deficient (Lrat(-/-)) mice, which lack hepatic retinoid stores.
Biochemical markers of liver injury, hepatic encephalopathy, oxidative stress, and xenobiotic-metabolizing enzyme activity were assessed.
The effect of exogenous retinyl acetate administration on TAA-induced hepatotoxicity in Lrat(-/-) mice was evaluated.

Main Results:

WT mice exhibited significant liver injury, hepatic encephalopathy, altered redox balance, and increased xenobiotic metabolism after TAA administration.
Lrat(-/-) mice showed no TAA-induced effects, indicating retinoid stores are crucial for TAA hepatotoxicity.
Supplementation with retinyl acetate induced TAA hepatotoxicity in Lrat(-/-) mice, confirming the role of retinoids.

Conclusions:

Endogenous hepatic retinoid stores play a critical role in modulating thioacetamide (TAA) hepatotoxicity in mice.
The findings highlight the significant impact of accumulated retinoids on the body's response to xenobiotic exposure.