Wenxinia saemankumensis sp. nov., isolated from a tidal flat sediment

A Gram-stain-negative, non-motile, ovoid- or rod-shaped bacterial strain, designated S-22(T), belonging to the class Alphaproteobacteria, was isolated from a tidal flat sediment of the Yellow Sea, Korea, and was subjected to a polyphasic taxonomic study. Strain S-22(T) grew optimally at pH 7.0-8.0, at 25 °C and in the presence of 2-3% (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences showed that strain S-22(T) joined Wenxinia marina HY34(T), with which it exhibited highest 16S rRNA gene sequence similarity (98.0%). The DNA G+C content was 72.3 mol% and the mean DNA-DNA relatedness value between strain S-22(T) and the type strain of W. marina was 3.7%. Strain S-22(T) contained Q-10 as the predominant ubiquinone and summed feature 8 (C(18 : 1)ω7c and/or C(18 : 1)ω6c) and C(16 : 0) as the major fatty acids. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified aminolipid, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, demonstrated that strain S-22(T) is distinguishable from W. marina. On the basis of the data presented, strain S-22(T) is considered to represent a novel species of the genus Wenxinia, for which the name Wenxinia saemankumensis sp. nov. is proposed. The type strain of is S-22(T) ( = KCTC 32548(T) = CECT 8456(T)).