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Flow metering characterization within an electrical cell counting microfluidic device.

U Hassan1, N N Watkins, C Edwards

  • 1William L. Everett Laboratory, Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, 1406 W. Green St., Urbana, IL 61801, USA.

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|March 12, 2014
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Summary
This summary is machine-generated.

This study presents integrated microfluidic methods for precise fluid flow characterization and sample volume metering. These advancements are crucial for accurate cell counting in microfluidic devices utilizing the Coulter principle.

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Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Cell Counting Technology

Background:

  • Microfluidic devices employing the Coulter principle necessitate precise aperture dimensions for accurate cell counting.
  • Accurate cell counting requires simultaneous metering of sample volume to determine absolute cell counts within a defined volume.
  • Existing microfluidic systems often lack integrated solutions for both fluid characterization and volume metering.

Purpose of the Study:

  • To develop and validate integrated methods for fluid flow characterization within microfluidic channels.
  • To demonstrate an electrically-based approach for precise sample volume metering in microfluidic devices.
  • To enhance the accuracy and applicability of Coulter principle-based cell counting systems.

Main Methods:

  • Characterization of fluid flow and impedance changes across a 15 μm × 15 μm Coulter aperture using lysed whole blood.
  • Electrically-based sample metering by analyzing impedance variations and cell passage events.
  • Utilizing leukocytes, erythrocyte cell lysate, and reagents for flow experiments.

Main Results:

  • A linear relationship was observed between fluid flow rate and the change in impedance across the measurement aperture.
  • Fluid volume was successfully determined by measuring decreased pulse width and increased cell count per unit time.
  • Demonstrated the feasibility of electrically-based sample metering in microfluidic channels.

Conclusions:

  • Integrated fluid flow characterization and sample metering are achievable in microfluidic devices.
  • The presented electrical methods offer a robust solution for volume determination in microfluidic cell counting.
  • These findings advance the development of sophisticated microfluidic diagnostic and analytical tools.