Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

5.8K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
5.8K
Mass Spectrometry: Alcohol Fragmentation01:03

Mass Spectrometry: Alcohol Fragmentation

4.7K
Alcohols (R-OH) ionize to lose one non-bonded electron from the oxygen atom, forming molecular ions. Due to their tendency to fragment rapidly, the intensity of the molecular ion peak in the mass spectrum is weak or sometimes absent. The fragmentation patterns for alcohols occur in two ways, i.e. ⍺-cleavage and dehydration. During ⍺-cleavage, the bond at the ⍺-position adjacent to the hydroxyl group cleaves to give a resonance-stabilized cation and a radical. However,...
4.7K
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

1.4K
Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
1.4K
Microbes in Beverage Production01:25

Microbes in Beverage Production

298
Alcoholic beverages such as wine, beer, and spirits are the products of microbial fermentation processes that transform simple sugars into ethanol and a wide array of complex flavor compounds. These transformations rely on the metabolic activities of specific yeasts and bacteria, which are selected and controlled to yield the desired beverage characteristics.Wine Fermentation and MaturationWine production begins with the crushing of grapes to release juice and pulp, forming a must that is...
298
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

3.0K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Lung transplantation, a contemporary review for the general parctitioner].

MMW Fortschritte der Medizin·2026
Same author

[Comparative analysis of drug therapy for fibrotic interstitial lung diseases based on results of phase 3 study].

Pneumologie (Stuttgart, Germany)·2026
Same author

Benchmarking DNA barcode decoding strategies under high error rates.

BMC bioinformatics·2026
Same author

Eosinophilic Pleural Effusion Following Long-Term Asthma Remission Under Dupilumab Therapy: A Search for Clues Using Cytokine Analyses.

Journal of investigational allergology & clinical immunology·2026
Same author

Prolyl-3-hydroxylase 1 is a central regulator of collagen post-translational modifications and the collagen biosynthetic network.

The Journal of biological chemistry·2026
Same author

Pulmonary hypertension associated with interstitial lung disease: a state-of-the-art review.

The European respiratory journal·2026

Related Experiment Video

Updated: May 2, 2026

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry
13:29

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry

Published on: May 28, 2014

23.4K

Wine yeast typing by MALDI-TOF MS.

Julia C Usbeck1, Caroline Wilde, Dave Bertrand

  • 1Lehrstuhl für Technische Mikrobiologie, Technische Universität München, Gregor-Mendel Str. 4, 85354, Freising, Germany.

Applied Microbiology and Biotechnology
|March 12, 2014
PubMed
Summary

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) offers a rapid method for differentiating Saccharomyces yeast strains. This technique aids in predicting yeast application potential, improving wine quality.

More Related Videos

Author Spotlight: Exploring the Fermentation Microbiome Through Next-Generation Sequencing
07:34

Author Spotlight: Exploring the Fermentation Microbiome Through Next-Generation Sequencing

Published on: December 1, 2023

1.5K
Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment Kartchner Caverns, AZ, USA
11:09

Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment Kartchner Caverns, AZ, USA

Published on: January 2, 2015

13.6K

Related Experiment Videos

Last Updated: May 2, 2026

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry
13:29

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry

Published on: May 28, 2014

23.4K
Author Spotlight: Exploring the Fermentation Microbiome Through Next-Generation Sequencing
07:34

Author Spotlight: Exploring the Fermentation Microbiome Through Next-Generation Sequencing

Published on: December 1, 2023

1.5K
Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment Kartchner Caverns, AZ, USA
11:09

Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment Kartchner Caverns, AZ, USA

Published on: January 2, 2015

13.6K

Area of Science:

  • Microbiology
  • Biotechnology
  • Food Science

Background:

  • Saccharomyces cerevisiae is crucial for wine production, with strain selection significantly impacting wine quality.
  • Differentiating yeast strains is essential for ensuring consistent beverage quality and characteristics in modern winemaking.

Purpose of the Study:

  • To evaluate matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as an alternative to traditional methods for yeast strain differentiation.
  • To assess the potential of MALDI-TOF MS for predicting yeast application in winemaking.

Main Methods:

  • Generated mass fingerprints of 33 Saccharomyces strains using MALDI-TOF MS with optimized sample preparation and instrument settings.
  • Performed cluster analysis on MALDI-TOF MS data for strain differentiation.
  • Utilized delta-PCR as a reference method to study genetic diversity and compare with MALDI-TOF MS results.

Main Results:

  • MALDI-TOF MS successfully differentiated Saccharomyces strains at the ecotype level.
  • Cluster analysis of MALDI-TOF MS data correlated well with genetic diversity assessed by delta-PCR.
  • MALDI-TOF MS provided insights into yeast strain relationships and application potential.

Conclusions:

  • MALDI-TOF MS is a viable, efficient alternative to biochemical and DNA-based methods for Saccharomyces strain differentiation in winemaking.
  • The proteome-level information from MALDI-TOF MS aids in understanding yeast relationships and predicting their suitability for specific wine fermentations.