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Corneal endothelial cell loss following trephination
T E Clinch1, K L Fung, P L Laibson
1Cornea Service, Wills Eye Hospital, Philadelphia, Pennsylvania 19107.
Ophthalmic Surgery
|October 1, 1988
Summary
Corneal trephination causes minimal endothelial cell loss, with peripheral loss at 7.9% and total loss at 3%. Both Weck and H&I corneal punches demonstrated comparable results in endothelial cell damage and removal during eye surgery.
Area of Science:
- Ophthalmology
- Corneal Surgery
- Tissue Engineering
Background:
- Endothelial cell loss is a critical concern in corneal transplantation.
- Accurate quantification of cell loss during surgical preparation is essential for graft survival.
Purpose of the Study:
- To evaluate endothelial cell loss during corneal trephination using two distinct corneal punch types.
- To compare the efficacy of Weck and H&I corneal punches in minimizing endothelial cell damage.
Main Methods:
- Utilized 40 porcine eyes for the study.
- Employed a vital staining technique with alizarin red S and acid violet 19.
- Quantified endothelial cell damage and removal following trephination with Weck and H&I corneal punches.
Main Results:
- Average peripheral endothelial cell loss was 7.9%.
- Total endothelial cell loss across the corneal button surface area was approximately 3%.
- No statistically significant difference (P > 0.1) in endothelial cell damage or removal was observed between Weck and H&I punches.
Conclusions:
- Corneal trephination results in a minor percentage of endothelial cell loss.
- Both Weck and H&I corneal punches are comparable in their impact on endothelial cells during donor cornea preparation.
- These findings support the use of either punch type with minimal concern for differential endothelial cell loss.