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R-spondins/Lgrs expression in tooth development.

Maiko Kawasaki1, Thantrira Porntaveetus, Katsushige Kawasaki

  • 1Department of Craniofacial Development and Stem Cell Biology, Dental Institute, King's College London, Guy's Hospital, London Bridge, London, United Kingdom; Division of Bio-Prosthodontics, Department of Oral Health Science, Course for Oral Life Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|March 12, 2014
PubMed
Summary

R-spondin/Lgr signaling regulates tooth development. R-spondin2 deficiency in mice leads to extra teeth in the diastema, indicating its role in controlling tooth formation.

Keywords:
LgrsR-spondinsdiastematooth development

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Area of Science:

  • Developmental Biology
  • Mammalian Tooth Development
  • Signaling Pathways

Background:

  • Tooth development relies on complex signaling pathways, including Wnt signaling.
  • R-spondins activate Wnt signaling, with Lgr4-6 acting as their receptors.
  • The specific role of R-spondin/Lgr signaling in tooth development is not well understood.

Purpose of the Study:

  • To investigate the role of R-spondin/Lgr signaling in mammalian tooth development.
  • To analyze the expression patterns of R-spondins and Lgrs during murine odontogenesis.

Main Methods:

  • Comparative in situ hybridization of R-spondins and Lgrs in murine tooth germs.
  • Analysis of tooth development in R-spondin2 mutant mice.

Main Results:

  • R-spondin2 was expressed in both tooth germs and the diastema.
  • R-spondin2 mutant mice showed no significant molar or incisor abnormalities.
  • Supernumerary teeth were observed in the diastema of R-spondin2 mutant mice.

Conclusions:

  • R-spondin/Lgr signaling plays a role in tooth development.
  • R-spondin2 is involved in regulating tooth formation, particularly in the diastema region.