Nucleotide sequence and polymorphism of MHC class II DQB exon 2 alleles in Chinese yakow (Bos grunniens × Bos taurus)
1Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, China.
Abstract:
The objective of this study is to describe the gene characteristics of the bovine lymphocyte antigen (BoLA)-DQB exon 2 locus in Chinese yakow (Bos grunniens × Bos taurus) and to compare it with previously reported patterns in other bovidae species to investigate genetic factors for disease resistance. The exon 2 of the MHC class II DQB gene was cloned and sequenced. It was revealed by sequence analyses that there are 36 DQB exon 2 alleles among 44 Chinese yakow. These alleles exhibited a high degree of nucleotide and amino acid polymorphism with most amino acid variations occurring at positions forming the peptide-binding sites (PBS). The DQB loci were analysed for patterns of synonymous (dS ) and nonsynonymous (dN ) substitution. The Chinese yakow was observed to be under strong positive selection in the DQB exon 2 peptide-binding sites (dN = 0.147, P < 0.01). It appears that this variability among Chinese yakow confers the ability to mount immune responses to a wide variety of peptides or pathogens.
More Related Videos
Related Concept Videos
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon...
Epistasis
Single Nucleotide Polymorphisms-SNPs
Incomplete Dominance
Modern Molecular Taxonomy
Comparing Copy Number Variations and SNPs
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...


