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Low molecular weight RNA of Drosophila cells which is induced by heat shock--II. Structural properties
Y Kawata1, H Fujiwara, T Shiba
1Department of Biology, College of Arts and Sciences, University of Tokyo, Japan.
Abstract:
Heat shock-induced small cytoplasmic RNA (HSI scRNA) from Drosophila culture cell was larger in size after dimethylchloroacetal treatment than in 7 M urea. Judging from its thermal denaturation profile and the nuclease S1 digestion experiment it was supposed that HSI scRNA is double-stranded. Nucleotide sequence analysis of the scRNA also suggested that it is double-stranded RNA with the blunt-ends.