Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Factors Influencing Drug Absorption: Disease States and Pharmacology01:25

Factors Influencing Drug Absorption: Disease States and Pharmacology

1.9K
Multiple disease states can significantly influence the oral drug absorption process by affecting blood flow and the functionality of the gastrointestinal (GI) system. Various GI diseases, including conditions that alter GI motility, such as diarrhea, decreased acid secretions (achlorhydria), and infections, have been associated with reduced drug absorption.
Substances such as alcohol and specific drugs, including antineoplastics, can also negatively impact drug absorption. For instance,...
1.9K
Inflammatory Bowel Disease III: Diagnostic Studies and Management I-Nutritional Therapy01:30

Inflammatory Bowel Disease III: Diagnostic Studies and Management I-Nutritional Therapy

1.1K
Various diagnostic tests are employed in the diagnostic process for Inflammatory Bowel Disease (IBD), particularly to differentiate between Crohn's disease and ulcerative colitis.
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
1.1K
Drugs for Treatment of Constipation-Predominant IBS01:21

Drugs for Treatment of Constipation-Predominant IBS

1.4K
Pharmacological therapies for IBS-C are designed to alleviate abdominal discomfort and enhance bowel function. In patients with IBS-C, fiber supplements may help soften stools and decrease straining, but may also lead to increased gas production and bloating. Osmotic laxatives like milk of magnesia are frequently used to soften stools and increase stool frequency in IBS-C patients. In addition, two drugs approved for use in severe IBS-C adult cases are linaclotide (Linzess) and lubiprostone...
1.4K
Lipid Absorption01:24

Lipid Absorption

3.9K
Dietary triglycerides from chyme in the duodenum are mixed with bile salts produced by the liver to emulsify fats. As a result, large droplets are broken down into smaller ones, increasing the surface area for enzymatic action. Once emulsified, pancreatic lipases hydrolyze the triglycerides into free fatty acids and monoglycerides.
These breakdown products bind with bile salts and lecithin to form micelles, which quickly pass between microvilli to come in close contact with the apical...
3.9K
Inflammatory Bowel Disease II: Ulcerative Colitis01:20

Inflammatory Bowel Disease II: Ulcerative Colitis

34
Ulcerative colitis is a chronic inflammatory disorder of the colon characterized by continuous mucosal inflammation that typically begins in the rectum and extends proximally in a uniform pattern. Its pathogenesis involves a complex interplay of genetic predisposition, immune dysregulation, and environmental influences. These factors converge to impair the colon’s epithelial defenses and promote an exaggerated inflammatory response against luminal contents.Breakdown of the Mucosal...
34
Drug Absorption: Factors Affecting GI Absorption01:19

Drug Absorption: Factors Affecting GI Absorption

6.2K
The process of oral drug absorption can be influenced by several factors. Weakly acidic drugs tend to be absorbed more readily from the stomach due to their nonionized state. However, absorption may be less efficient in the upper intestine, where drugs are often ionized. Interestingly, despite the stomach's apparent advantage for drug absorption, its mucous layer can hinder diffusion. Its surface area is also smaller than the intestine's, which can further slow down the absorption rate.
6.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Endothelial MerTK impairment promotes cardiac dysfunction in the condition of high fat diet.

Redox biology·2026
Same author

<i>Dnmt3b</i> Deficiency in Adipocyte Progenitor Cells Ameliorates Obesity in Female Mice.

International journal of molecular sciences·2026
Same author

Engineering an orthogonal ubiquitin transfer cascade with RING E3 RNF38 by phage display to reveal its regulation of nuclear transport.

The Journal of biological chemistry·2026
Same author

GHSR suppression in neurons protects against aging-associated metabolic and cognitive impairments.

GeroScience·2025
Same author

Endothelial MerTK impairment accelerates the development of atherosclerosis.

Redox biology·2025
Same author

Epigenetic programming of estrogen receptor in adipocytes by high-fat diet regulates obesity-induced inflammation.

JCI insight·2025

Related Experiment Video

Updated: May 2, 2026

The Isolation of Flowing Mesenteric Lymph in Mice to Quantify In Vivo Kinetics of Dietary Lipid Absorption and Chylomicron Secretion
06:14

The Isolation of Flowing Mesenteric Lymph in Mice to Quantify In Vivo Kinetics of Dietary Lipid Absorption and Chylomicron Secretion

Published on: November 30, 2022

3.4K

Intestinal Cgi-58 deficiency reduces postprandial lipid absorption.

Ping Xie1, Feng Guo2, Yinyan Ma3

  • 1Department Of Biochemistry, Wake Forest University Health Sciences, Winston-Salem, North Carolina, United States of America; Institute Of Medicinal Plant Development, Chinese Academy Of Medical Sciences & Peking Union Medical College, Beijing, China.

Plos One
|March 13, 2014
PubMed
Summary

Comparative Gene Identification-58 (CGI-58) protein is crucial for intestinal fat metabolism. Its absence in mice impairs triglyceride breakdown, leading to fat accumulation and altered lipid profiles, impacting metabolic health.

More Related Videos

Evaluating Therapeutic Interventions in the SHIP-deficient Mouse Model of Crohn Disease-like Ileitis and Fibrosis
09:44

Evaluating Therapeutic Interventions in the SHIP-deficient Mouse Model of Crohn Disease-like Ileitis and Fibrosis

Published on: October 14, 2025

631
A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids
10:12

A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids

Published on: October 13, 2019

8.3K

Related Experiment Videos

Last Updated: May 2, 2026

The Isolation of Flowing Mesenteric Lymph in Mice to Quantify In Vivo Kinetics of Dietary Lipid Absorption and Chylomicron Secretion
06:14

The Isolation of Flowing Mesenteric Lymph in Mice to Quantify In Vivo Kinetics of Dietary Lipid Absorption and Chylomicron Secretion

Published on: November 30, 2022

3.4K
Evaluating Therapeutic Interventions in the SHIP-deficient Mouse Model of Crohn Disease-like Ileitis and Fibrosis
09:44

Evaluating Therapeutic Interventions in the SHIP-deficient Mouse Model of Crohn Disease-like Ileitis and Fibrosis

Published on: October 14, 2025

631
A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids
10:12

A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids

Published on: October 13, 2019

8.3K

Area of Science:

  • Lipid Metabolism
  • Molecular Biology
  • Gastroenterology

Background:

  • Comparative Gene Identification-58 (CGI-58) is a lipid droplet (LD)-associated protein known to promote triglyceride (TG) hydrolysis.
  • Human CGI-58 mutations lead to TG accumulation in various tissues, including the intestine.
  • Enterocytes, the cells lining the intestine, are not typically known to store TG-rich LDs, but transient accumulation occurs after fatty meals.

Purpose of the Study:

  • To investigate the role of CGI-58 in intestinal lipid metabolism and its impact on postprandial lipid handling.
  • To identify the proteins responsible for LD-TG hydrolysis in enterocytes.
  • To establish an animal model for studying intestinal fat metabolism in metabolic disorders.

Main Methods:

  • Generation of intestine-specific CGI-58 knockout mice.
  • Analysis of plasma and intestinal triglyceride concentrations postprandially.
  • Measurement of intestinal TG hydrolase activity, fatty acid absorption, oxidation, and cholesterol levels.

Main Results:

  • Intestine-specific CGI-58 inactivation significantly reduced postprandial plasma TG and intestinal TG hydrolase activity.
  • Knockout mice exhibited a 4-fold increase in intestinal TG content and LD accumulation in enterocytes.
  • Decreased intestinal fatty acid absorption/oxidation and altered plasma/intestinal/hepatic cholesterol levels were observed.

Conclusions:

  • Intestinal CGI-58 is essential for efficient postprandial lipoprotein-TG secretion and maintaining overall lipid homeostasis.
  • The study provides a valuable mouse model for investigating intestinal fat metabolism's role in obesity and type 2 diabetes.
  • CGI-58 plays a critical role in regulating intestinal lipid droplet hydrolysis and managing systemic lipid levels.