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Related Concept Videos

Proteomics01:33

Proteomics

7.5K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Pathophysiology of Heart Failure01:17

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Heart failure (HF) is a progressive syndrome involving ventricles that leads to inadequate cardiac output. It can be classified based on location and output or ejection fraction. Ejection fraction (EF) is an essential measurement in the diagnosis and surveillance of HF. Reduced EF corresponds to systolic heart failure (HFrEF). However, HF with preserved ejection fraction (HFpEF) is becoming increasingly prevalent. Also known as diastolic HF, this form of HF is related to aging. The...
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Heart Failure II: Pathophysiology01:29

Heart Failure II: Pathophysiology

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Systolic Heart Failure and Compensatory MechanismsSystolic heart failure (also termed HFrEF, Heart Failure with Reduced Ejection Fraction) is the most prevalent type of heart filure. It results in a decreased volume of blood being pumped from the ventricle. The aortic arch and carotid sinuses have baroreceptors that detect reduced blood pressure, triggering the sympathetic nervous system (SNS) to release epinephrine and norepinephrine. Initially, this response aims to boost heart rate and...
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Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
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Proteomics in heart failure: top-down or bottom-up?

Zachery R Gregorich1, Ying-Hua Chang, Ying Ge

  • 1Molecular and Cellular Pharmacology Training Program, University of Wisconsin-Madison, Madison, WI, USA.

Pflugers Archiv : European Journal of Physiology
|March 13, 2014
PubMed
Summary

Understanding heart failure (HF) requires studying molecular pathways. Mass spectrometry (MS) proteomics, using bottom-up and top-down strategies, offers powerful insights into HF's cellular mechanisms and protein functions.

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Area of Science:

  • Biochemistry and Molecular Biology
  • Cardiovascular Research
  • Proteomics

Background:

  • Heart failure (HF) pathophysiology is complex, involving diverse etiologies and cellular process aberrations.
  • Understanding molecular pathways driving the HF phenotype is crucial for advancing treatment strategies.
  • Proteins are key effector molecules, directly influencing cell function and phenotype.

Purpose of the Study:

  • To review the advantages and disadvantages of mass spectrometry (MS)-based proteomics for heart failure research.
  • To highlight the contributions of bottom-up and top-down proteomics to understanding HF mechanisms.
  • To discuss challenges and future directions in MS-based proteomics for HF.

Main Methods:

  • Comparison of peptide-based bottom-up and protein-based top-down mass spectrometry (MS) strategies.
  • Analysis of protein identification, quantification, and post-translational modifications using MS.
  • Review of existing literature on MS-based proteomics applications in heart failure.

Main Results:

  • Both bottom-up and top-down MS have unique strengths and weaknesses for proteome interrogation.
  • These proteomics strategies have significantly advanced the understanding of molecular and cellular mechanisms in HF.
  • Specific applications include protein identification, quantification, and analysis of post-translational modifications relevant to HF.

Conclusions:

  • Mass spectrometry-based proteomics is essential for systems-level understanding of heart failure pathophysiology.
  • Both bottom-up and top-down approaches are valuable tools, offering complementary insights into HF.
  • Future research should address current challenges to further leverage MS-based proteomics in HF.