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Updated: May 2, 2026

A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells
Published on: October 28, 2025
Assignments of RNase A by ADAPT-NMR and enhancer
Marco Tonelli1, Chelcie H Eller, Kiran K Singarapu
1National Magnetic Resonance Facility at Madison, University of Wisconsin - Madison, 433 Babcock Drive, Madison, WI, 53706, USA, tonelli@nmrfam.wisc.edu.
Abstract:
We report here backbone (1)H and (15)N assignments for ribonuclease A obtained by using ADAPT-NMR, a fully-automated approach for combined data collection, spectral analysis and resonance assignment. ADAPT-NMR was able to assign 98% of the resonances with 93% agreement with traditional data collection and assignment. Further refinement of the automated results with ADAPT-NMR enhancer led to complete (100%) assignments with 96% agreement with assignments by the traditional approach.

