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CSF-1R expression in tumor-associated macrophages is associated with worse prognosis in classical Hodgkin lymphoma
Young Wha Koh1, Chansik Park, Dok Hyun Yoon
1Dept of Pathology, University of Ulsan College of Medicine, Asan Medical Center, 88, Olympic-ro 43-gil, Seoul 138-736, South Korea; jrhuh@amc.seoul.kr.
Insights
High colony-stimulating 1 receptor (CSF-1R) expression in non-Hodgkin/Reed-Sternberg (non-HRS) cells indicates a worse prognosis for classical Hodgkin lymphoma (CHL) patients. This correlates with increased tumor macrophages and poorer survival outcomes.
Area of Science:
- Oncology
- Immunology
- Pathology
Background:
- Classical Hodgkin lymphoma (CHL) is a malignancy of lymphocytes.
- The tumor microenvironment, including macrophages, plays a role in lymphoma progression.
- Colony-stimulating 1 receptor (CSF-1R) is involved in macrophage regulation.
Purpose of the Study:
- To investigate the prognostic significance of CSF-1R expression in both malignant (HRS) and non-malignant (non-HRS) cells in CHL.
- To correlate CSF-1R expression with tumor-associated macrophage (TAM) infiltration.
- To assess the impact of CSF-1R expression on patient survival outcomes.
Main Methods:
- Retrospective analysis of diagnostic tissues from 112 CHL patients.
- Immunohistochemical assessment of CSF-1R, CD68, and CD163 expression.
- Correlation of CSF-1R expression levels with event-free survival (EFS) and overall survival (OS).
Main Results:
- High CSF-1R expression (≥30%) in non-HRS cells was linked to inferior EFS and OS.
- Elevated non-HRS cell CSF-1R expression correlated significantly with increased TAMs (CD163+).
- Coexpression of CSF-1R and CD163 in non-HRS cells predicted the worst survival outcomes.
Conclusions:
- Increased CSF-1R expression in non-HRS cells is a marker of poor prognosis in CHL.
- This finding is associated with a higher density of tumor-associated macrophages.
- CSF-1R expression in the tumor microenvironment warrants further investigation for therapeutic targeting.
Objectives:
The aim of this study was to determine the prognostic relevance of colony-stimulating 1 receptor (CSF-1R) expression in both Hodgkin/Reed-Sternberg (HRS) cells and the surrounding cells (non-HRS cells) in patients with classical Hodgkin lymphoma (CHL) .
Methods:
Diagnostic tissues from 112 patients with CHL treated with doxorubicin, bleomycin, vinblastine, and dacarbazine were evaluated retrospectively by immunohistochemical analysis for CSF-1R and CD68 and CD163 for tissue-associated macrophages.
Results:
High numbers (≥30%) of non-HRS cells expressing CSF-1R conferred inferior event-free survival and overall survival in univariate and multivariate analysis. High numbers of non-HRS cells expressing CSF-1R were significantly associated with a high number of tumor-associated macrophages as detected by CD163 expression (P < .001). In particular, coexpression of CSF-1R and CD163 was associated with a worse survival outcome than either CSF-1R or CD163 expression alone or no expression.
Conclusions:
Our data demonstrate that a high number of non-HRS cells expressing CSF-1R are correlated with an increased tumor macrophage content and worse survival.
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