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Updated: Aug 14, 2026
The Mouse Round-window Approach for Ototoxic Agent Delivery: A Rapid and Reliable Technique for Inducing Cochlear Cell Degeneration
Published on: November 26, 2015
Microanatomic analysis of the round window membrane by white light interferometry and microcomputed tomography for
Hirobumi Watanabe1, Jeffrey W Kysar, Anil K Lalwani
1*Department of Otolaryngology, New York University School of Medicine; †Department of Mechanical Engineering, Columbia University; and ‡Department of Otolaryngology, Columbia University College of Physicians and Surgeons, New York, New York, U.S.A.
Objective:
The round window membrane (RWM) is increasingly becoming a target for amplification using active middle ear implants. However, the current strategy of using available transducer tips may have negative consequences for the RWM. We investigated the microanatomy of the RWM to establish a basis for the design of the transducer tip for the RWM driver.
Study Design:
Using the guinea pig as an animal model, microcomputed tomography (μCT) and white light interferometry were used to study the topography of the RWM and RW niche (RWN). The curvatures of the RWM surface were calculated using the topography data.
Main Outcome Measures:
The 3-dimensional structure of the scala tympani terminal, saddle-shaped surface topography, and surface curvature were determined.
Results:
The size of the scala terminal was approximated as an ellipse for which the major and minor axes were 1.29 and 0.95 mm. The average minimum and maximum radii of curvature around the center of RWM were -0.44 and +0.70 mm along the minor and major axis.
Conclusion:
The microanatomies of the RWM and RWN have important implications for the design of the transducer tip to maximize energy transfer while minimizing its distortion and permanent disruption. Our results suggest that the size of the transducer tip should be smaller than the minor axis of the scala terminal to avoid collision with the RWN. The driver should be designed to conform to the topography and radius of curvature of the center portion of the RWM, which for a guinea pig is 0.44 mm.
