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Comparative efficacy of Belzer or Euro-Collins solutions for pancreatic preservation during cold ischemic storage in
Rogério Renato Perez1, Alberto Goldenberg2, Alcides Augusto Salzedas Netto3
1Sao Paulo Federal University, Sao PauloSP, Brazil, Fellow Master degree, Postgraduate Program in Interdisciplinary Surgical Sciences, Sao Paulo Federal University(UNIFESP), Sao Paulo-SP, Brazil. Surgical procedures, acquisition and interpretation of data, manuscript writing.
Purpose:
To compare the efficacy of different types of solutions (Belzer or Euro-Collins) for the preservation of rat pancreas during cold ischemia.
Methods:
Thirty Wistar rats were divided into three groups according to the perfusion or storage solution: Group E (perfusion and storage in Euro-Collins solution); Group B (perfusion and storage in Belzer solution) and Group BE (Perfusion in Belzer solution and storage in Euro-Collins solution). After perfusion, the pancreas was excised and stored at 4˚C for 18 hours. Amylase was measured at 6, 12 and 18h, and histological analysis of the pancreas was performed after 18h of cold storage.
Results:
Amylase was elevated and comparable in Groups E and BE after 12 and 18 hours of ischemia (p<0.05). In the exocrine pancreas, histological differences in the amount of necrosis (p=0.049), lymphocytic infiltrate (p<0.001) and neutrophilic infiltrate (p=0.004) were observed, with more favorable features present in Group B. In the endocrine pancreas, Group B showed less edema (p<0.001), but other parameters were similar among all groups.
Conclusion:
The Euro-Collins solution is inferior to the Belzer solution for the preservation of rat pancreas during cold ischemia.
